Application
Western blot analysis of GALT expression in non-transfected: (A) and human GALT transfected: (B) 293T whole cell lysates.Application
Western blot analysis of GALT expression in non-transfected: (A) and human GALT transfected: (B) 293 whole cell lysates and human liver tissue extract (C).| Product Name | GALT Antibody (H-300) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 29-328 mapping within an internal region of GALT of human origin |
| Modification Notes | GALT (galactose-1-phosphate uridylyltransferase) is a 379 amino acid member of the galactose-1-phosphate uridylyltransferase type 1 family of proteins. GALT exists as a homodimer and is believed to play a role in galactose metabolism. More specifically, GALT is responsible for catalyzing the reaction of UDP-glucose with å-D-galactose 1-phosphate to produce å-D-glucose 1-phosphate and UDP-galactose. This is the second step of the Leloir pathway of galactose metabolism. The products of this reaction will either enter the glycolytic pathway to yield energy (å-D-glucose 1-phosphate) or be used as a galactosyl donor in the synthesis of glycoproteins and glycolipids (UDP-galactose). Mutations in the gene encoding GALT can lead to galactosemia, a disorder (occurring from the inability to metabolize galactose) that is characterized by cataracts, mental retardation and jaundice. In newborns, galactosemia can be fatal if lactose is not removed from the diet. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of GALT of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine and porcine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 29-328 mapping within an internal region of GALT of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 29-328 mapping within an internal region of GALT of human origin |
Application
Western blot analysis of GALT expression in non-transfected: (A) and human GALT transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of GALT expression in non-transfected: (A) and human GALT transfected: (B) 293 whole cell lysates and human liver tissue extract (C).| Application Notes | recommended for detection of GALT of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine and porcine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
