Application
Western blot analysis of GNPDA1 expression in non-transfected: (A) and human GNPDA1 transfected: (B) 293T whole cell lysates.Application
Western blot analysis of GNPDA1 expression in MIA PaCa-2 (A) and COLO 320DM (B) whole cell lysates.| Product Name | GNPDA1 Antibody (FL-289) |
|---|---|
| Antibody Type | Primary Antibodies |
| Modification Notes | During fertilization in mammals, the sperm activates the egg by causing an increase in the level of free cytoplasmic calcium concentration. This increased calcium concentration induces a characteristic series of oscillations that trigger egg activation and early embryo development. A hamster protein named oscillin is thought to be involved in this pathway. The enzyme glucosamine-6-phosphate isomerase (GNPI) or deaminase (GNPDA1) and the related protein GNPDA2 are the human homologs of hamster oscillin. GNPDA1 and GNPDA2 catalyze the conversion of GNP to fructose-6-phosphate and ammonia. Both proteins exist as homohexamers and are ubiquitously expressed with highest expression in testis, ovary and heart. Three isoforms of GNPDA2 are expressed due to alternative splicing events. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of GNPDA1 of mouse, rat and human origin by WB, IP, IF and ELISA may cross-react with GNPDA2 also reactive with additional species, including equine, canine, bovine, porcine and avian | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 1-289 representing full length GNPDA1 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 1-289 representing full length GNPDA1 of human origin |
| Entrez Gene | 10007 |
|---|
Application
Western blot analysis of GNPDA1 expression in non-transfected: (A) and human GNPDA1 transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of GNPDA1 expression in MIA PaCa-2 (A) and COLO 320DM (B) whole cell lysates.| Application Notes | recommended for detection of GNPDA1 of mouse, rat and human origin by WB, IP, IF and ELISA may cross-react with GNPDA2 also reactive with additional species, including equine, canine, bovine, porcine and avian |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
