Application
Western blot analysis of TGFβ1 expression in rat adrenal gland (A) and mouse uterus (B) tissue extracts.Application
Western blot analysis of TGFβ1 expression in 293T whole cell lysate.Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded mouse ovary tissue showing extracellular localization.Application
Western blot analysis of TGFβ1 expression in rat adrenal (A) and mouse uterus (B) extracts and MCF7 (C) and T-47D (D) whole cell lysates.Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded porcine uterus tissue. Kindly provided by Laurie A. Jaeger.| Product Name | TGFβ1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Conjugation | Agarose |
| Modification | Acetylated |
| Modification Notes | Transforming growth factor betas (TGF∫s) were originally discovered due to their ability to promote anchorage-independent growth of rat NRK fibroblasts in the presence of TGFå. It is now realized that TGF∫s mediate many cell-cell interactions that occur during embryonic development. Three TGF∫s have been identified in mammals . TGF∫1, TGF∫2 and TGF∫3 are each synthesized as precursor proteins that are very similar in that each is cleaved to yield a 112 amino acid polypeptide that remains associated with the latent portion of the molecules. Biologically active TGF∫ requires dimerization of the monomers (usually homodimers) and release of the latent peptide portion. Overall, the mature region of the TGF∫3 protein has approximately 80% identity to the mature region of both TGF∫1 and TGF∫2. However, the NH2 terminals or precursor regions of their molecules share only 27% sequence identity. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFIHCIPWB |
| recommended for detection of precursor and mature TGFβ1, and to a lesser extent, TGFβ2 of mouse, rat, human and Xenopus laevis origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, canine, bovine, porcine and avian: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Target | epitope mapping at the C-terminus of TGFβ1 of human origin |
|---|---|
| Tissue Specificity | epitope mapping at the C-terminus of TGFβ1 of human origin |
| Entrez Gene | 7040 |
|---|
Application
Western blot analysis of TGFβ1 expression in rat adrenal gland (A) and mouse uterus (B) tissue extracts.
Application
Western blot analysis of TGFβ1 expression in 293T whole cell lysate.
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded mouse ovary tissue showing extracellular localization.
Application
Western blot analysis of TGFβ1 expression in rat adrenal (A) and mouse uterus (B) extracts and MCF7 (C) and T-47D (D) whole cell lysates.
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded porcine uterus tissue. Kindly provided by Laurie A. Jaeger.| Application Notes | recommended for detection of precursor and mature TGFβ1, and to a lesser extent, TGFβ2 of mouse, rat, human and Xenopus laevis origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, canine, bovine, porcine and avian: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
