Application
Western blot analysis of Haptoglobin α expression in human PBL whole cell lysate.| Product Name | Haptoglobin α Antibody (H-130) |
|---|---|
| Antibody Type | Primary Antibodies |
| Modification Notes | Haptoglobin (Hp) is a blood plasma protein that functions to bind free Hemoglobin that has been released from erythrocytes, thereby inhibiting its oxidative activity. During this process, Haptoglobin sequesters the iron within Hemoglobin, preventing iron-utilizing bacteria from benefitting from hemolysis. This function suggests that Haptoglobin concentrations may increase in response to inflammation. The resulting Haptoglobin-Hemoglobin complex is then removed by the reticulo-endothelial system. Due to cleavage of a common precursor protein during protein synthesis, Haptoglobin consists of two å and two ∫ chains, connected by disulfide bridges. In human, Haptoglobin exists in two allelic forms designated Haptoglogin 1 (Hp1) and Haptoglobin 2 (Hp2), where Hp2 is the result of a partial Hp1 gene duplication. There are three known phenotypes of human Haptoglobin: Hp1-1, Hp2-1 and Hp2-2, which may be associated with diabetes and cardiovascular disease pathology and a susceptibility to Parkinson’s and Crohn’s disease. Haptoglobin levels are useful in diagnosing hemolytic anemia, the abnormal breakdown of red blood cells. Haptoglobin is expressed in mammalian hepatocytes as well as other tissues such as skin, lung and kidney. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of Haptoglobin &alpha of human and, to a lesser extent, mouse and rat origin by WB, IP, IF and ELISA also recommended for detection of Haptoglobin-related protein (HRP) of human origin | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 21-150 mapping near the N-terminus of Haptoglobin of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 21-150 mapping near the N-terminus of Haptoglobin of human origin |
| Entrez Gene | 3240 |
|---|
Application
Western blot analysis of Haptoglobin α expression in human PBL whole cell lysate.| Application Notes | recommended for detection of Haptoglobin &alpha of human and, to a lesser extent, mouse and rat origin by WB, IP, IF and ELISA also recommended for detection of Haptoglobin-related protein (HRP) of human origin |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
