Application
Western blot analysis of AFAP-1L1 expression in non-transfected: (A) and mouse AFAP-1L1 transfected: (B) 293T whole cell lysates.Application
Western blot analysis of AFAP-1L1 expression in non-transfected: (A) and mouse AFAP-1L1 transfected: (B) 293T whole cell lysates.Application
Western blot analysis of human recombinant AFAP-1L1 fusion protein.| Product Name | AFAP-1L1 Antibody (H-105) |
|---|---|
| Antibody Type | Primary Antibodies |
| Modification Notes | Actin filament associated protein (AFAP-110) interacts directly with actin filaments through its C-terminal actin-binding domain. AFAP-110 contains additional protein-binding domains as well, and serves as an adaptor protein. By linking signaling molecules to actin filaments, AFAP-110 provides a platform for the preparation of larger signaling complexes, activates Src kinases in response to cellular signals and also directly affects Actin organization as an Actin filament cross-linking protein. AFAP-1L1 (Actin filament-associated protein 1-like 1) is a 768 amino acid protein that, like its relative AFAP110, contains two Pleckstrin homology (PH) domains, which are normally found in proteins involved in intracellular signaling. AFAP-1L1 is phosphorylated upon DNA damage, probably by ATR or Atm. There are four isoforms of AFAP-1L1 that are produced as a result of alternative splicing events. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of AFAP-1L1 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine and porcine | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 651-755 mapping near the C-terminus of AFAP-1L1 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 651-755 mapping near the C-terminus of AFAP-1L1 of human origin |
Application
Western blot analysis of AFAP-1L1 expression in non-transfected: (A) and mouse AFAP-1L1 transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of AFAP-1L1 expression in non-transfected: (A) and mouse AFAP-1L1 transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of human recombinant AFAP-1L1 fusion protein.| Application Notes | recommended for detection of AFAP-1L1 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine and porcine |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
