Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human bronchus tissue showing cytoplasmic staining of respiratory epithelial cells.Application
Western blot analysis of Centrin-1/2 expression in mouse testis tissue extract.| Product Name | Centrin-1/2 Antibody (H-40) |
|---|---|
| Antibody Type | Primary Antibodies |
| Modification Notes | EF-hand type Ca2+-binding proteins consists of several family members, including Centrin-1, Centrin-2 and Centrin-3. The Centrin proteins are ubiquitously expressed cytoskeletal components that show increased expression during cell differentiation. Tissues where cilia are present, such as the retina and testis, express both Centrin-1 and -2, but Centrin-2 is also expressed in nondifferentiated, nonciliated retinal cells (retinoblastoma cells), liver, skeletal muscle, and cardiac muscle. In these tissues, Centrin associates with the centrosomes, mitotic spindle poles, and basal bodies. Knockdown studies reveal a requirement for Centrin in centriole duplication and organization of spindle pole morphology and the completion of cytokinesis. Centrin-3 plays a role in centrosome reproduction. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| recommended for detection of Centrin-1 and Centrin-2 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA may cross-react with other Centrins also reactive with additional species, including equine, canine, bovine, porcine and avian | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 93-132 mapping within an internal region of Centrin-2 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 93-132 mapping within an internal region of Centrin-2 of human origin |
| Entrez Gene | 1068 |
|---|
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human bronchus tissue showing cytoplasmic staining of respiratory epithelial cells.
Application
Western blot analysis of Centrin-1/2 expression in mouse testis tissue extract.| Application Notes | recommended for detection of Centrin-1 and Centrin-2 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA may cross-react with other Centrins also reactive with additional species, including equine, canine, bovine, porcine and avian |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
