Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human thyroid tissue showing cytoplasmic staining of glandular cells.Application
Western blot analysis of IL-23 expression in non-transfected: (A) and mouse IL-23 transfected: (B) 293T whole cell lysates.| Product Name | IL-23 Antibody (H-113) |
|---|---|
| Antibody Type | Primary Antibodies |
| Modification Notes | The p19 protein shares sequence similarity with IL-6 subfamily members and is distantly related to the p35 subunit of IL-12 (1). p19 shows no biological activity by itself; instead, it combines with the p40 subunit of IL-12 to form a biologically active, composite cytokine, IL-23 (1). IL-23 shares some in vivo functions with IL-12, including the activation of the transcription factor Stat4 (2). Indeed, the receptors for each appear to share one subunit, but also have at least one distinct subunit (2). Activated dendritic cells secrete detectable levels of this heterodimeric complex and IL-23 binds to IL-12R beta 1 but fails to engage IL-12R beta 2 (1,2). Similar to IL-12, human IL-23 stimulates IFN-g production and proliferation in PHA blast T cells, as well as in CD45RO (memory) T cells (1,4). Ubiquitous transgenic expression of the IL-23 subunit p19 induces multiorgan inflammation, runting, infertility, and premature death (3,4). The gene which encodes IL-23 maps to human chromosome 12 (5). |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| recommended for detection of IL-23 &alpha subunit p19 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, canine, bovine, porcine and avian | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 75-187 mapping near the C-terminus of IL-23 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 75-187 mapping near the C-terminus of IL-23 of human origin |
| Entrez Gene | 51561 |
|---|
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human thyroid tissue showing cytoplasmic staining of glandular cells.
Application
Western blot analysis of IL-23 expression in non-transfected: (A) and mouse IL-23 transfected: (B) 293T whole cell lysates.| Application Notes | recommended for detection of IL-23 &alpha subunit p19 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, canine, bovine, porcine and avian |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
