Application
Immunofluorescence staining of formalin-fixed Hep G2 cells showing cytoplasmic localization.| Product Name | NUDC Antibody (H-271) |
|---|---|
| Antibody Type | Primary Antibodies |
| Modification Notes | NUDC (nuclear distribution gene C homolog, A. nidulans), also known as HNUDC, MNUDC or NPD011, is a ubiquitously expressed protein that is conserved from fungus to human. Highly expressed in proliferating cells, NUDC localizes to the cytoplasm and nucleus, contains a CS domain and participates in neurogenesis, mitosis, nuclear migration and cytokinesis. At the onset of mitosis, NUDC is phosphorylated by Plk. This modification of NUDC is required for proper mitotic spindle formation, chromosome separation during mitosis, cytokinesis and cell proliferation. In neurons and fibroblasts, NUDC forms a complex with LIS1 that localizes to the microtubule network and microtubule-organizing center and facilitates nuclear movement and transport in migrating neurons. In addition, the NUDC/LIS1 complex can associate with the minus-end directed Dynein/Dynactin motor complex and, together, these complexes cooperate in the regulation of cytokinesis. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of NUDC of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine and porcine | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 61-331 mapping at the C-terminus of NUDC of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 61-331 mapping at the C-terminus of NUDC of human origin |
| Entrez Gene | 10726 |
|---|
Application
Immunofluorescence staining of formalin-fixed Hep G2 cells showing cytoplasmic localization.| Application Notes | recommended for detection of NUDC of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine and porcine |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
