Application
Western blot analysis of involucrin expression in BC3H1 (A), NIH/3T3 (B) and MDCK (C) whole cell lysate.IF/ICC
CK5 and involucrin were red, while the nuclei were blue (a).WB
The western blot results revealed that the expression of CK5 and involucrin was upregulated in the miR-203a-3p mimic group compared with the mimic NC group, while it was downregulated in the miR-203a-3p inhibitor group compared with the inhibitor NC group (b)WB
Western blotting showed that the expression of CK5 and involucrin was upregulated after transfection of short interfering siSmad1-003.| Product Name | involucrin Antibody (M-116) |
|---|---|
| Antibody Type | Primary Antibodies |
| Modification Notes | Involucrin is a precursor protein of the keratinocyte cornified envelope, which is formed beneath the inner surface of the cell membrane during terminal differentiation. Involucrin first appears in the cell cytosol but ultimately becomes cross-linked to membrane proteins by transglutaminase. During keratinocyte terminal differentiation, glutamine residues of involucrin become covalently cross-linked to other envelope precursors via covalent epsilon-(gamma-glutamyl) lysine bonds. Moreover, its large size allows involucrin to cross-link molecules that are separated by substantial distances in the cornified envelope. These properties allow a single involucrin molecule to form multiple cross-links, in multiple spatial planes, with other envelope precursors. Involucrin is specifically expressed in Chinese hamster ovarian cells (fibroblasts), PtK2 rat kangaroo kidney cells (simple epithelial), and rat epidermal keratinocytes (stratifying squamous epithelial). |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAICC/IFIPWB |
| recommended for detection of involucrin of mouse and rat origin by WB, IP, IF and ELISA | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 5-120 mapping at the N-terminus of involucrin of mouse origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 5-120 mapping at the N-terminus of involucrin of mouse origin |
| Entrez Gene | 16447 |
|---|
Application
Western blot analysis of involucrin expression in BC3H1 (A), NIH/3T3 (B) and MDCK (C) whole cell lysate.
IF/ICC
CK5 and involucrin were red, while the nuclei were blue (a).
WB
The western blot results revealed that the expression of CK5 and involucrin was upregulated in the miR-203a-3p mimic group compared with the mimic NC group, while it was downregulated in the miR-203a-3p inhibitor group compared with the inhibitor NC group (b)
WB
Western blotting showed that the expression of CK5 and involucrin was upregulated after transfection of short interfering siSmad1-003.| Application Notes | recommended for detection of involucrin of mouse and rat origin by WB, IP, IF and ELISA |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
