Application
Immunofluorescence staining of formalin-fixed Hep G2 cells showing cytoskeletal localization.Application
Western blot analysis of CLAMP expression in mouse testis tissue extract (A) and Hep G2 whole cell lysate (B).| Product Name | CLAMP Antibody (E-14) |
|---|---|
| Antibody Type | Primary Antibodies |
| Modification Notes | CLAMP, also known as SPEF1 (sperm flagellar protein 1), is a 236 amino acid protein that is present in epididymal sperm and localizes to the cell projection, as well as to the cytoplasm. Expressed in lung, brain and testis, CLAMP functions as a microtubule-associated protein that is thought to play a role in microtubule bundling. Human CLAMP exists as two alternatively spliced isoforms and shares a high degree of homology with its mouse counterpart, suggesting a conserved role between species. The gene encoding CLAMP maps to human chromosome 20, which houses over 600 genes and comprises nearly 2% of the human genome. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of CLAMP of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine and porcine | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope mapping within an internal region of CLAMP of human origin |
|---|---|
| Tissue Specificity | epitope mapping within an internal region of CLAMP of human origin |
| Entrez Gene | 25876 |
|---|
Application
Immunofluorescence staining of formalin-fixed Hep G2 cells showing cytoskeletal localization.
Application
Western blot analysis of CLAMP expression in mouse testis tissue extract (A) and Hep G2 whole cell lysate (B).| Application Notes | recommended for detection of CLAMP of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine and porcine |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
