Application
Western blot analysis of ATE1 expression in Hep G2 (A), MDA-MB-231 (B) and Jurkat (C) whole cell lysates.| Product Name | ATE1 Antibody (H-300) |
|---|---|
| Antibody Type | Primary Antibodies |
| Modification Notes | Arginyl-tRNA-protein transferase (ATE1), also designated arginyltransferase 1, belongs to the R-transferase family of proteins. In order for a protein to be degraded via the ubiquitin pathway, arginylation of the protein is required. ATE1 plays an important role in this process, as it is important for the posttranslational conjugation of arginine to the N-terminal aspartate-, glutamate- and possibly cystine-contaning substrates. ATE1 is a 518 amino acid protein. Alternative splicing results in two distinct isoforms. ATE1, which is found as a monomer, can localize to the cytoplasm and/or the nucleus. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of ATE1 isoforms 1 and 2 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine and porcine | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 1-300 mapping at the N-terminus of ATE1 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 1-300 mapping at the N-terminus of ATE1 of human origin |
Application
Western blot analysis of ATE1 expression in Hep G2 (A), MDA-MB-231 (B) and Jurkat (C) whole cell lysates.| Application Notes | recommended for detection of ATE1 isoforms 1 and 2 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine and porcine |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
