Application
Western blot analysis of IleRS expression in Ramos whole cell lysate.Application
Western blot analysis of IleRS expression in Caki-1 (A) and HeLa (B) whole cell lysates.| Product Name | IleRS Antibody (H-219) |
|---|---|
| Antibody Type | Primary Antibodies |
| Modification Notes | The fidelity of protein synthesis requires efficient discrimination of amino acid substrates by aminoacyl-tRNA synthetases. Accurate discrimination of the structurally similar amino acids, valine and isoleucine, by isoleucyl-tRNA synthetase (IleRS) results, in part, from a hydrolytic editing reaction, which prevents misactivated valine from being stably joined to tRNAIle. IleRS joins Ile to tRNA(Ile) at its synthetic active site and hydrolyzes incorrectly acylated amino acids at its editing active site. A member of the aminoacyl-tRNA synthetase family, human IleRS has been identified as a target of antibodies in the autoimmune disease polymyositis. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of IleRS of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine and bovine | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 781-999 mapping within an internal region of IleRS of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 781-999 mapping within an internal region of IleRS of human origin |
| Entrez Gene | 3376 |
|---|
Application
Western blot analysis of IleRS expression in Ramos whole cell lysate.
Application
Western blot analysis of IleRS expression in Caki-1 (A) and HeLa (B) whole cell lysates.| Application Notes | recommended for detection of IleRS of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine and bovine |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
