Application
Western blot analysis of TGFβ2 expression in non-transfected: (A) and mouse TGFβ2 transfected: (B) 293T whole cell lysates.Application
Immunofluorescence staining of normal mouse intestine frozen section showing cytoplasmic staining.Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human small intestine tissue showing cytoplasmic and extracellular localization.Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human placenta tissue showing cytoplasmic and extracellular localization.Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded porcine small intestine tissue. Kindly provided by Laurie A. Jaeger.Application
Western blotting analysis of 10 ng each of human recombinant TGFβ1 (A), TGFβ2 (B) and TGFβ3 (C).Application
Western blot analysis of human recombinant TGFβ2.| Product Name | TGFβ2 (V) Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Modification Notes | Transforming growth factor betas (TGF∫s) were originally discovered due to their ability to promote anchorage-independent growth of rat NRK fibroblasts in the presence of TGFå. It is now realized that TGF∫s mediate many cell-cell interactions that occur during embryonic development. Three TGF∫s have been identified in mammals. TGF∫1, TGF∫2 and TGF∫3 are each synthesized as precursor proteins that are very similar in that each is cleaved to yield a 112 amino acid polypeptide that remains associated with the latent portion of the molecules. Biologically active TGF∫ requires dimerization of the monomers (usually homodimers) and release of the latent peptide portion. Overall, the mature region of the TGF∫3 protein has approximately 80% identity to the mature region of both TGF∫1 and TGF∫2. However, the NH2 terminals or precursor regions of their molecules share only 27% sequence identity. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| recommended for detection of TGF&beta2 and, to a lesser extent, TGF&beta3 of mouse, rat, human, porcine and Xenopus origin by WB, IP, IF, IHC(P) and ELISA non-cross reactive with TGF&beta1 also reactive with additional species, including equine, canine, bovine, porcine and avian | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope mapping at the C-terminus of TGF&beta 2 of human origin |
|---|---|
| Tissue Specificity | epitope mapping at the C-terminus of TGFβ2 of human origin |
| Entrez Gene | 7042 |
|---|
Application
Western blot analysis of TGFβ2 expression in non-transfected: (A) and mouse TGFβ2 transfected: (B) 293T whole cell lysates.
Application
Immunofluorescence staining of normal mouse intestine frozen section showing cytoplasmic staining.
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human small intestine tissue showing cytoplasmic and extracellular localization.
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human placenta tissue showing cytoplasmic and extracellular localization.
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded porcine small intestine tissue. Kindly provided by Laurie A. Jaeger.
Application
Western blotting analysis of 10 ng each of human recombinant TGFβ1 (A), TGFβ2 (B) and TGFβ3 (C).
Application
Western blot analysis of human recombinant TGFβ2.| Application Notes | recommended for detection of TGF&beta2 and, to a lesser extent, TGF&beta3 of mouse, rat, human, porcine and Xenopus origin by WB, IP, IF, IHC(P) and ELISA non-cross reactive with TGF&beta1 also reactive with additional species, including equine, canine, bovine, porcine and avian |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
