Application
Immunofluorescence staining of methanol-fixed HeLa cells showing cytoskeletal localization.Application
Western blot analysis of Vimentin expression in HISM whole cell lysate. Note lower molecular weight Vimentin degradation products.Application
Immunofluorescence staining of methanol-fixed HeLa cells showing cytoplasmic localization.| Product Name | Vimentin Antibody (H-84) |
|---|---|
| Antibody Type | Primary Antibodies |
| Modification Notes | Cytoskeletal intermediate filaments (IFs) constitute a diverse group of proteins that are expressed in a highly tissue-specific manner. Intermediate filaments are constructed from two-chain, a-helical, coiled-coil molecules arranged on an imperfect helical lattice and have been widely used as markers for distinguishing individual cell types within a tissue and identifying the origins of metastatic tumors. One such intermediate filament protein, Vimentin, is a general marker of cells originating in the mesenchyme. Vimentin is frequently coexpressed with other members of the intermediate filament family, such as the cytokeratins, in neoplasms including melanoma and breast carcinoma. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of Vimentin of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including canine and porcine | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 1-84 mapping at the N-terminus of Vimentin of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 1-84 mapping at the N-terminus of Vimentin of human origin |
| Entrez Gene | 7431 |
|---|
Application
Immunofluorescence staining of methanol-fixed HeLa cells showing cytoskeletal localization.
Application
Western blot analysis of Vimentin expression in HISM whole cell lysate. Note lower molecular weight Vimentin degradation products.
Application
Immunofluorescence staining of methanol-fixed HeLa cells showing cytoplasmic localization.| Application Notes | recommended for detection of Vimentin of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including canine and porcine |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
