Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human appendix tissue showing nuclear staining of glandular and lymphoid cells at low (A) and high (B) magnification. Kindly provided by The Swedish Human Protein Atlas (HPA) program.Application
Western blot analysis of Sp2 expression in non-transfected: (A) and human Sp2 transfected: (B) 293T whole cell lysates.Application
Immunofluorescence staining of normal mouse liver frozen section showing nuclear staining.Application
Immunofluorescence staining of methanol-fixed K-562 cells showing nuclear staining.Application
Western blot analysis of Sp2 expression in K-562 nuclear extract.| Product Name | Sp2 Antibody (K-20) |
|---|---|
| Antibody Type | Primary Antibodies |
| Modification Notes | The Sp transcription factor family includes Sp1, Sp2, Sp3 (SPR-2) and Sp4 (SPR-1). Sp transcription factors share similar structures but do not share similar functions. All four proteins contain a highly conserved DNA-binding domain composed of three zinc fingers at the C-terminus. Sp family members bind the consensus sequence GGGGCGGGGC and other closely related sequences which are known as GC boxes. Sp1, Sp3 and Sp4 share a high affinity for GC boxes while Sp2 does not. Sp2 only weakly binds to GT boxes. Sp1, Sp2 and Sp3 are ubiquitously expressed, while Sp4 is abundantly expressed in brain with limited expression in other tissues. Sp1 and Sp3, but not Sp2 or Sp4, interact with E2, a regulatory element for the b4 subunit of neuronal nicotinic acetylcholine receptors. Sp3 is the only Sp member to inhibit Sp1 and Sp4 mediated transcription. The gene3 encoding human Sp2 maps to chromosome 17p32.3. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISAGSChIP |
| recommended for detection of Sp2 by mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, canine, bovine, porcine and avian | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope mapping near the N-terminus of Sp2 of human origin |
|---|---|
| Tissue Specificity | epitope mapping near the N-terminus of Sp2 of human origin |
| Entrez Gene | 6668 |
|---|
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human appendix tissue showing nuclear staining of glandular and lymphoid cells at low (A) and high (B) magnification. Kindly provided by The Swedish Human Protein Atlas (HPA) program.
Application
Western blot analysis of Sp2 expression in non-transfected: (A) and human Sp2 transfected: (B) 293T whole cell lysates.
Application
Immunofluorescence staining of normal mouse liver frozen section showing nuclear staining.
Application
Immunofluorescence staining of methanol-fixed K-562 cells showing nuclear staining.
Application
Western blot analysis of Sp2 expression in K-562 nuclear extract.| Application Notes | recommended for detection of Sp2 by mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, canine, bovine, porcine and avian |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
