Application
Antibodies tested include p-CREB-1 (Ser 133)-R: (A,B,C) and CREB-1 (C-21): (D,E,F).Application
Antibodies tested include p-CREB-1 (Ser 133): (A,B,C) and CREB-1 (C-21): (D,E,F).Application
Immunofluorescence staining of methanol-fixed HeLa cells showing nuclear and cytoplasmic localization.Application
Antibodies tested include p-CREB-1 (Ser 133)-R: (A,B) and CREB-1 (C-21): (C,D).Application
Western blot analysis of CREB-1 expression in Jurkat nuclear extract.Application
Western blot analysis of CREB-1 expression in non-transfected: (A) and mouse CREB-1 transfected: (B) 293T whole cell lysates.Application
Western blot analysis of CREB-1 expression in non-transfected: (A) and human CREB-1 transfected: (B) 293 whole cell lysates.| Product Name | CREB-1 Antibody (C-21) |
|---|---|
| Antibody Type | Primary Antibodies |
| Modification Notes | Eukaryotic gene transcription is regulated by sequence-specific transcription factors that bind modular cis-acting promoter and enhancer elements. The ATF/CREB transcription factor family binds the palindromic cAMP response element (CRE) octanucleotide TGACGTCA. The ATF/CREB family includes CREB-1, CREB-2 (also designated ATF-4), ATF-1, ATF-2 and ATF-3. This family of proteins contains highly divergent N-terminal domains, but shares a C-terminal leucine zipper for dimerization and DNA binding. Although CREB can bind to DNA in an unphosphorylated state, it cannot activate transcription. Phosphorylation of CREB on Ser 133 by protein kinase A facilitates its interaction with the CREB-binding protein (CBP) and activates the basal transcription complex. CREB functions in neoglucogenesis through interactions with the nuclear coactivator PGC-1. CREB may play a role in the pathogenesis of type II diabetes and dilated cardiomyopathy. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISAGSChIP |
| recommended for detection of CREB-1A, CREB-1B, CREM and ATF-1 of mouse, rat, human and Drosophila origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine, porcine and avian | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope mapping near the C-terminus of CREB-1 of human origin |
|---|---|
| Tissue Specificity | epitope mapping near the C-terminus of CREB-1 of human origin |
| Entrez Gene | 466 |
|---|
Application
Antibodies tested include p-CREB-1 (Ser 133)-R: (A,B,C) and CREB-1 (C-21): (D,E,F).
Application
Antibodies tested include p-CREB-1 (Ser 133): (A,B,C) and CREB-1 (C-21): (D,E,F).
Application
Immunofluorescence staining of methanol-fixed HeLa cells showing nuclear and cytoplasmic localization.
Application
Antibodies tested include p-CREB-1 (Ser 133)-R: (A,B) and CREB-1 (C-21): (C,D).
Application
Western blot analysis of CREB-1 expression in Jurkat nuclear extract.
Application
Western blot analysis of CREB-1 expression in non-transfected: (A) and mouse CREB-1 transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of CREB-1 expression in non-transfected: (A) and human CREB-1 transfected: (B) 293 whole cell lysates.| Application Notes | recommended for detection of CREB-1A, CREB-1B, CREM and ATF-1 of mouse, rat, human and Drosophila origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine, porcine and avian |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
