Application
Western blot analysis of Ferrochelatase expression in Jurkat (A), K-562 (B) and HEL 92.1.7 (C) whole cell lysates.Application
Immunofluorescence staining of methanol-fixed HeLa cells showing cytoplasmic and nuclear localization.| Product Name | Ferrochelatase Antibody (H-300) |
|---|---|
| Antibody Type | Primary Antibodies |
| Modification Notes | Ferrochelatase, also designated Heme synthetase or protoheme ferro-lyase, is the terminal enzyme of protoheme biosynthesis that catalyzes the ferrous form of iron insertion into protoporphyrin IX. Mature ferrochelatase is a homodimeric, mitochondrial membrane-associated protein translated downstream of an N-terminal 54-amino acid transit peptide. Ferrochelatase contains two nitric oxide (NO)-sensitive clusters and coordinated 2FE-2S clusters which may potentially serve as a nitric oxide sensor. Defects in the gene encoding the Ferrochelatase enzyme, FECH, cause erythropoietic protoporhyria (EPP), which is a dominantly inherited disease of porphyrin metabolism characterized by photosensitivity and hepatobiliary disease. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of mitochondrial precursor and mature Ferrochelatase of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine, porcine and avian | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 124-423 mapping at the C-terminus of Ferrochelatase of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 124-423 mapping at the C-terminus of Ferrochelatase of human origin |
| Entrez Gene | 2235 |
|---|
Application
Western blot analysis of Ferrochelatase expression in Jurkat (A), K-562 (B) and HEL 92.1.7 (C) whole cell lysates.
Application
Immunofluorescence staining of methanol-fixed HeLa cells showing cytoplasmic and nuclear localization.| Application Notes | recommended for detection of mitochondrial precursor and mature Ferrochelatase of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine, porcine and avian |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
