Application
Western blot analysis of Sp2 expression in non-transfected: (A) and human Sp2 transfected: (B) 293T whole cell lysates.Application
Western blot analysis of Sp2 expression in A-673 nuclear extract.| Product Name | Sp2 Antibody (H-282) |
|---|---|
| Antibody Type | Primary Antibodies |
| Modification Notes | The Sp transcription factor family includes Sp1, Sp2, Sp3 (SPR-2) and Sp4 (SPR-1). Sp transcription factors share similar structures but do not share similar functions. All four proteins contain a highly conserved DNA-binding domain composed of three zinc fingers at the C-terminus. Sp family members bind the consensus sequence GGGGCGGGGC and other closely related sequences which are known as GC boxes. Sp1, Sp3 and Sp4 share a high affinity for GC boxes while Sp2 does not. Sp2 only weakly binds to GT boxes. Sp1, Sp2 and Sp3 are ubiquitously expressed, while Sp4 is abundantly expressed in brain with limited expression in other tissues. Sp1 and Sp3, but not Sp2 or Sp4, interact with E2, a regulatory element for the b4 subunit of neuronal nicotinic acetylcholine receptors. Sp3 is the only Sp member to inhibit Sp1 and Sp4 mediated transcription. The gene3 encoding human Sp2 maps to chromosome 17q21.32. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISAGSChIP |
| recommended for detection of Sp2 of human origin by WB, IP, IF and ELISA also reactive with additional species, including bovine and canine | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 154-435 of Sp2 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 154-435 of Sp2 of human origin |
| Entrez Gene | 6668 |
|---|
Application
Western blot analysis of Sp2 expression in non-transfected: (A) and human Sp2 transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of Sp2 expression in A-673 nuclear extract.| Application Notes | recommended for detection of Sp2 of human origin by WB, IP, IF and ELISA also reactive with additional species, including bovine and canine |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
