Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human lung tissue showing cytoplasmic staining of macrophages and pneumocytes.Application
Western blot analysis of GAPDH expression in non-transfected 293T: (A), human GAPDH transfected 293T: (B) and Hep G2 (C) whole cell lysates.Application
Western blot analysis of GAPDH expression in non-transfected 293T: (A), mouse GAPDH transfected 293T: (B) and HeLa (C) whole cell lysates.Application
Western blot analysis of GAPDH expression in Hep G2 whole cell lysate.Application
Western blot analysis of GAPDH expression in non-transfected 293T: (A), human GAPDH transfected 293T: (B) and A549 (C) whole cell lysates.Application
Western blot analysis of GAPDH expression in non-transfected 293T: (A), human GAPDH transfected 293T: (B) and KNRK (C) whole cell lysates.Application
Western blot analysis of GAPDH expression in JAR (A) and HeLa (B) whole cell lysates.Application
Immunofluorescence staining of methanol-fixed HeLa cells showing cytoplasmic localization using indirect FITC (A) staining and direct Alexa Fluor 488 (B) staining.Application
Western blot analysis of GAPDH expression in HeLa (A) and JAR (B) whole cell lysates and mouse placenta tissue extract (C).Application
Immunofluorescence staining of methanol-fixed HeLa cells showing cytoplasmic localization.| Product Name | GAPDH Antibody (FL-335) HRP |
|---|---|
| Antibody Type | Primary Antibodies |
| Modification Notes | Glyceraldehyde-3-phosphate dehydrogenase (GAPDH), also called uracil DNA glycosylase, catalyzes the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate in the presence of inorganic phosphate and nicotinamide adenine dinucleotide (NAD), an important energy-yielding step in carbohydrate metabolism. While GAPDH has long been recognized as playing an integral role in glycolysis, additional functions of GAPDH include acting as a uricil DNA glycosylase, activating transcription, binding RNA and involvement in nuclear RNA export, DNA replication and DNA repair. Expression of GAPDH is upregulated in liver, lung and prostate cancers. GAPDH translocates to the nucleus during apoptosis. GAPDH complexes with neuronal proteins implicated in human neuro-degenerative disorders including the b-Amyloid precursor, Huntingtin and other triplet repeat neuronal disorder proteins. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| recommended for detection of GAPDH and GAPDH-2 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | raised against amino acids 1-335 representing full length GAPDH of human origin |
|---|---|
| Tissue Specificity | raised against amino acids 1-335 representing full length GAPDH of human origin |
| Entrez Gene | 2597 |
|---|
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human lung tissue showing cytoplasmic staining of macrophages and pneumocytes.
Application
Western blot analysis of GAPDH expression in non-transfected 293T: (A), human GAPDH transfected 293T: (B) and Hep G2 (C) whole cell lysates.
Application
Western blot analysis of GAPDH expression in non-transfected 293T: (A), mouse GAPDH transfected 293T: (B) and HeLa (C) whole cell lysates.
Application
Western blot analysis of GAPDH expression in Hep G2 whole cell lysate.
Application
Western blot analysis of GAPDH expression in non-transfected 293T: (A), human GAPDH transfected 293T: (B) and A549 (C) whole cell lysates.
Application
Western blot analysis of GAPDH expression in non-transfected 293T: (A), human GAPDH transfected 293T: (B) and KNRK (C) whole cell lysates.
Application
Western blot analysis of GAPDH expression in JAR (A) and HeLa (B) whole cell lysates.
Application
Immunofluorescence staining of methanol-fixed HeLa cells showing cytoplasmic localization using indirect FITC (A) staining and direct Alexa Fluor 488 (B) staining.
Application
Western blot analysis of GAPDH expression in HeLa (A) and JAR (B) whole cell lysates and mouse placenta tissue extract (C).
Application
Immunofluorescence staining of methanol-fixed HeLa cells showing cytoplasmic localization.| Application Notes | recommended for detection of GAPDH and GAPDH-2 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
