Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human cervix tissue showing nuclear and cytoplasmic staining of epithelial cells.Application
Immunofluorescence staining of methanol-fixed T-47D cells showing nuclear localization.Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human breast tumor showing nuclear localization.Application
Antibodies tested include ERα (MC-20): , ERα (H-184): , CBP (A-22): , CBP (C-1): , p/CIP (F2): , p/CIP (M-397): , RIP140 (H-300): and HDAC3 (N-19): . Data kindly provided by M.G. Rosenfeld and reproduced with permission from Perissi et al., Cell 2004, 116: 511-526.Application
Western blot analysis of ERα expression in MCF7 whole cell lysate.Application
Western blot analysis of human recombinant ERα.| Product Name | ERα Antibody (H-184) |
|---|---|
| Antibody Type | Primary Antibodies |
| Modification Notes | Estrogen receptors (ER) are members of the steroid/thyroid hormone receptor superfamily of ligand-activated transcription factors. Estrogen receptors, including ERå and ER∫, contain DNA binding and ligand binding domains and are critically involved in regulating the normal function of reproductive tissues. They are located in the nucleus , though some estrogen receptors associate with the cell surface membrane and can be rapidly activated by exposure of cells to estrogen. ERå and ER∫ have been shown to be differentially activated by various ligands. Receptor-ligand interactions trigger a cascade of events, including dissociation from heat shock proteins, receptor dimerization, phosphorylation and the association of the hormone activated receptor with specific regulatory elements in target genes. Evidence suggests that ERå and ER∫ may be regulated by distinct mechanisms even though they share many functional characteristics. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISAGSChIP |
| recommended for detection of estrogen receptor &alpha of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, canine, bovine and porcine | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 2-185 mapping at the N-terminus of estrogen receptor &alpha of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 2-185 mapping at the N-terminus of estrogen receptor α of human origin |
| Entrez Gene | 2099 |
|---|
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human cervix tissue showing nuclear and cytoplasmic staining of epithelial cells.
Application
Immunofluorescence staining of methanol-fixed T-47D cells showing nuclear localization.
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human breast tumor showing nuclear localization.
Application
Antibodies tested include ERα (MC-20): , ERα (H-184): , CBP (A-22): , CBP (C-1): , p/CIP (F2): , p/CIP (M-397): , RIP140 (H-300): and HDAC3 (N-19): . Data kindly provided by M.G. Rosenfeld and reproduced with permission from Perissi et al., Cell 2004, 116: 511-526.
Application
Western blot analysis of ERα expression in MCF7 whole cell lysate.
Application
Western blot analysis of human recombinant ERα.| Application Notes | recommended for detection of estrogen receptor &alpha of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, canine, bovine and porcine |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
