Application
Western blot analysis of ASK 1 phosphorylation in non-transfected: (A) and human ASK 1 transfected: (B) 293T whole cell lysates.| Product Name | p-ASK 1 Antibody (Ser 83) |
|---|---|
| Antibody Type | Primary Antibodies |
| Modification Notes | Mitogen-activated protein (MAP) kinase cascades are activated by various extracellular stimuli including growth factors. The MEK kinases (also called MAP kinase kinase kinases) phosphorylate and activate the MAP kinases, including ERK, JNK and p38. The MEK kinases characterized to date include Raf-1, Raf-B, MOS, MEK kinase-1, MEK kinase-2, MEK kinase-3, MEK kinase-4 and ASK 1 (also designated MEK kinase-5). MEK kinase-1 has been shown to phosphorylate MEK-1 via a Raf-independent pathway. Evidence suggests that MEK-3 is preferentially activated by MEK kinase-3 and that MEK-4 is activated by both MEK kinase-2 and MEK kinase-3. MEK kinase-4 has been shown to specifically activate the JNK pathway. ASK 1 activates both MEK-4 and MEK-3/MEK-6 pathways. ASK 1 serine 83 is phosphorylated by Akt. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of Ser 83 phosphorylated ASK 1 of human origin by WB, IP, IF and ELISA | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to phosphorylated Ser 83 of ASK 1 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to phosphorylated Ser 83 of ASK 1 of human origin |
| Entrez Gene | 4217 |
|---|
Application
Western blot analysis of ASK 1 phosphorylation in non-transfected: (A) and human ASK 1 transfected: (B) 293T whole cell lysates.| Application Notes | recommended for detection of Ser 83 phosphorylated ASK 1 of human origin by WB, IP, IF and ELISA |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
