Application
Immunofluorescence staining of methanol-fixed Hela cells showing cytoplasmic localization.Application
Western blot analysis of TCP-1 γ expression in mouse testis (A) and rat testis (B) tissue extracts.| Product Name | TCP-1 γ Antibody (H-300) |
|---|---|
| Antibody Type | Primary Antibodies |
| Modification Notes | The protein TCP-1 (t complex polypeptide 1) is a subunit of the hetero-oligomeric complex CCT (chaperonin containing TCP-1) present in the eukaryotic cytosol. The CCT of eukaryotic cytosol is composed of eight different subunit species, TCP-1 å, ∫, ©, ∂, é, Ω, eta and theta, each encoded by a different gene. Two Ω subunits have been described: TCP-1 Ω (also designated TCP-1 Ω1) and TCP-1 Ω2. TCP-1 subunits are proposed to have independent functions in folding its in vivo substrates, the actins and tubulins. TCP-1 was first identified in the mouse as relevant for tail-less and embryonic lethal phenotypes. Sequences homologous to TCP-1 have been isolated in several other species, and the yeast TCP-1 has been shown to encode a molecular chaperone for Actin and Tubulin. TCP-1 found in mammalian cells and yeast plays an important role in the folding of cytosolic proteins. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of TCP-1 &gamma of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine and porcine | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 101-400 mapping within an internal region of TCP-1 &gamma of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 101-400 mapping within an internal region of TCP-1 γ of human origin |
| Entrez Gene | 7203 |
|---|
Application
Immunofluorescence staining of methanol-fixed Hela cells showing cytoplasmic localization.
Application
Western blot analysis of TCP-1 γ expression in mouse testis (A) and rat testis (B) tissue extracts.| Application Notes | recommended for detection of TCP-1 &gamma of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine and porcine |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
