Application
Western blot analysis of PDC-E2 expression in non-transfected: (A) and mouse PDC-E2 transfected: (B) 293T whole cell lysates.Application
Western blot analysis of PDC-E2 expression in non-transfected: (A) and mouse PDC-E2 transfected: (B) 293T whole cell lysates.Application
Western blot analysis of PDC-E2 expression in non-transfected 293T: (A), mouse PDC-E2 transfected 293T: (B) and HeLa (C) whole cell lysates.Application
Western blot analysis of PDC-E2 expression in non-transfected 293T: (A), human PDC-E2 transfected 293T: (B) and HeLa (C) whole cell lysates.Application
Immunofluorescence staining of normal mouse intestine frozen section showing cytoplasmic staining.Application
Immunofluorescence staining of methanol-fixed HeLa cells showing cytoplasmic localization.| Product Name | PDC-E2 Antibody (H-160) |
|---|---|
| Antibody Type | Primary Antibodies |
| Modification Notes | Primary biliary cirrhosis (PBC) is a chronic, destructive autoimmune liver disease characterized by the presence of antimitochondrial autoantibodies in patient’s serum and T cell-mediated destruction of the biliary epithelial cells lining the small intrahepatic bile ducts. Patient sera are characterized by a high frequency (greater than 95%) of autoantibodies directed to a mitochondrial antigen, identified as the E2 component of the pyruvate dehydrogenase multienzyme complex (PDC-E2). PDC-E2 contains both an amino-terminal lipoyl-bearing domain and a carboxy-terminal catalytic domain. The human sequence preserves the Glu-Thr-Asp-Lys-Ala motif of the lipoyl-bearing site. Two conformationally alternative forms of the PDC-E2 protein have been revealed by immunoblotting. The immunodominant autoepitopes of the autoantigens correspond to the inner lipoyl domain. A significant number of asymptomatic patients found to have antibodies to PDC-E2 are at high risk of developing primary biliary cirrhosis. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of PDC-E2 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including canine, bovine and porcine | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 231-390 of PDC-E2 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 231-390 of PDC-E2 of human origin |
| Entrez Gene | 1737 |
|---|
Application
Western blot analysis of PDC-E2 expression in non-transfected: (A) and mouse PDC-E2 transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of PDC-E2 expression in non-transfected: (A) and mouse PDC-E2 transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of PDC-E2 expression in non-transfected 293T: (A), mouse PDC-E2 transfected 293T: (B) and HeLa (C) whole cell lysates.
Application
Western blot analysis of PDC-E2 expression in non-transfected 293T: (A), human PDC-E2 transfected 293T: (B) and HeLa (C) whole cell lysates.
Application
Immunofluorescence staining of normal mouse intestine frozen section showing cytoplasmic staining.
Application
Immunofluorescence staining of methanol-fixed HeLa cells showing cytoplasmic localization.| Application Notes | recommended for detection of PDC-E2 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including canine, bovine and porcine |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
