Application
Western blot analysis of CRBP I expression in non-transfected: (A) and mouse CRBP I transfected: (B) 293T whole cell lysates.Application
Western blot analysis of CRBP I expression in non-transfected: (A) and mouse CRBP I transfected: (B) 293T whole cell lysates.Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human testis tissue showing cytoplasmic staining of Leydig cells and cells in seminiferus ducts at low (A) and high (B) magnification. Kindly provided by The Swedish Human Protein Atlas (HPA) program.Application
Western blot analysis of CRBP I expression in mouse eye (A) and rat eye (B) tissue extracts.Application
Western blot analysis of CRBP I expression in SCC-4 whole cell lysate.| Product Name | CRBP I Antibody (FL-135) |
|---|---|
| Antibody Type | Primary Antibodies |
| Modification Notes | The cellular retinol-binding proteins (CRBP I, II, III and IV) belong to a superfamily of small cytoplasmic proteins which interact with hydrophobic ligands. Vitamin A, a molecule essential for cell growth and differentiation, embryonic development and vision, is transported into the cell by the CRBPs in its alcoholic form, called retinol. Both CRBP I and II are composed of ten antiparallel ∫-strands, which form a ∫-barrel that contains the retinol molecule, and two å-helices, which cover the open ends of the barrel. CRBP I mediates the cellular uptake of retinol, solubilizes and detoxifies it for further transport within the cytoplasm, and presents it to the appropriate enzymes to biosynthesize retinoic acid, an active form of retinol or retinyl esters, which are stored. CRBP I is expressed in human ovary, adrenal and pituitary glands, and testis, and its expression is modulated by TGF∫. CRBP II is expressed solely in the small intestine and mediates the absorption of retinoids and carotenoids to biosynthesize retinyl esters. CRBP III and CRBP IV are cytoplasmic proteins that, like CRBP I and CRBP II, form ∫-barrel structures and participate in the intracellular transport of retinol. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| recommended for detection of CRBP I partially cross-reactive with other CRBP family members of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, bovine, porcine and avian | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 1-135 representing full length CRBP I of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 1-135 representing full length CRBP I of human origin |
| Entrez Gene | 5947 |
|---|
Application
Western blot analysis of CRBP I expression in non-transfected: (A) and mouse CRBP I transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of CRBP I expression in non-transfected: (A) and mouse CRBP I transfected: (B) 293T whole cell lysates.
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human testis tissue showing cytoplasmic staining of Leydig cells and cells in seminiferus ducts at low (A) and high (B) magnification. Kindly provided by The Swedish Human Protein Atlas (HPA) program.
Application
Western blot analysis of CRBP I expression in mouse eye (A) and rat eye (B) tissue extracts.
Application
Western blot analysis of CRBP I expression in SCC-4 whole cell lysate.| Application Notes | recommended for detection of CRBP I partially cross-reactive with other CRBP family members of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, bovine, porcine and avian |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
