Application
Immunofluorescence staining of methanol-fixed HeLa cells showing membrane localization.Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded endometrium tissue showing cytoplasmic staining of cells in endometrial stroma and glandular cells at low (A) and high (B) magnification). Kindly provided by The Swedish Human Protein Atlas (HPA) program.Application
Western blot analysis of mature GDF-8 expression in mouse embryo (A), rat skeletal muscle (B) and rat brain (C) tissue extracts.| Product Name | GDF-8/11 Antibody (H-109) |
|---|---|
| Antibody Type | Primary Antibodies |
| Modification Notes | Growth/differentiation factors (GDFs) are members of the TGF superfamily. Members of the TGF superfamily are involved in embryonic development and adult tissue homeostasis. GDF-8, also known as myostatin, has been shown to be a negative regulator of skeletal muscle growth. GDF-11 has been shown to control anterior/posterior patterning of the axial skeleton, and also regulates kidney and pancreas organogenesis. GDF-11 controls anterior/posterior patterning of the axial skeleton, regulates organogenesis by controlling the expression of GDNF, contributes to the control of HOX gene expression and induces phosphorylation of Smad2. In addition, GDF-11 mediates signaling of Nodal during left-right patterning and development of head structures and inhibits generation of new neurons by neuronal progenitors in the olfactory epithelium. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| recommended for detection of GDF-8 and GDF-11 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, canine, bovine, porcine and avian | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 267-375 of GDF-8 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 267-375 of GDF-8 of human origin |
| Entrez Gene | 2660 |
|---|
Application
Immunofluorescence staining of methanol-fixed HeLa cells showing membrane localization.
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded endometrium tissue showing cytoplasmic staining of cells in endometrial stroma and glandular cells at low (A) and high (B) magnification). Kindly provided by The Swedish Human Protein Atlas (HPA) program.
Application
Western blot analysis of mature GDF-8 expression in mouse embryo (A), rat skeletal muscle (B) and rat brain (C) tissue extracts.| Application Notes | recommended for detection of GDF-8 and GDF-11 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, canine, bovine, porcine and avian |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
