Application
Western blot analysis of RPA194 expression in non-transfected: (A) and truncated human RPA194 transfected: (B) 293T whole cell lysates.Application
Western blot analysis of RPA194 expression in KNRK (A) and HeLa (B) nuclear extracts. Note presence of proteolytically cleaved forms.| Product Name | RPA194 Antibody (H-300) |
|---|---|
| Antibody Type | Primary Antibodies |
| Modification Notes | RNA polymerases transcribe nuclear genes for ribosomal RNA, thus representing ribosomal biogenesis. RNA polymerase I (Pol I) is located in the nucleolus and transcribes class I genes, which code for large ribosomal RNA. Different subunits of the Pol I transcription machinery are targets of various physiological stimuli, which suggests that multiple signaling pathways are involved in carrying out Pol I transcription. RPA40 and RPA16 are subunits of Pol I that associate with each other at an early stage of RNA polymerase I assembly. RPA40 is essential for the function and integrity of the complex and is also an essential subunit of RNA polymerase III (Pol III). RPA40, RPA16 and RPA135 encode the three subunits of RNA polymerase I, respectively. RPA194 is the largest subunit of RNA Pol I and is not a component of Pol II and Pol III. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of RPA194 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine and porcine | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 1-300 mapping at the N-terminus of RPA194 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 1-300 mapping at the N-terminus of RPA194 of human origin |
| Entrez Gene | 25885 |
|---|
Application
Western blot analysis of RPA194 expression in non-transfected: (A) and truncated human RPA194 transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of RPA194 expression in KNRK (A) and HeLa (B) nuclear extracts. Note presence of proteolytically cleaved forms.| Application Notes | recommended for detection of RPA194 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine and porcine |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
