Application
Immunofluorescence staining of formalin-fixed Hep G2 cells showing nuclear localization.Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human cerebral cortex tissue showing nuclear staining of neuronal and non-neuronal cells at low (A) and high (B) magnification. Kindly provided by The Swedish Human Protein Atlas (HPA) program.Application
Western blot analysis of PSF expression in HeLa (A), A-431 (B), NIH/3T3 (C), 3611-RF (D), Hep G2 (E) and A-673 (F) nuclear extracts.| Product Name | PSF Antibody (H-80) |
|---|---|
| Antibody Type | Primary Antibodies |
| Modification Notes | Pre-mRNA splicing is a critical step in the post-translational regulation of gene expression. The process of removing intron sequences from mRNA is a two-step enzymatic reaction that requires the action of the spliceosome, a large multicomponent ribonucleoprotein complex. The polypyrimidine tract-binding protein (PTB)-associated splicing factor (PSF) is a ubiquitously expressed protein that forms a complex with PTB, also designated hnRNP I, which is required for early spliceosome formation and is essential for catalytic step II. The PSF protein contains two RNA recognition motifs (RRMs), a proline- and glutamine-rich amino terminal domain, and two carboxy-terminal nuclear localization signals. PSF is localized to the nucleus in punctate structures called speckles, which are absent from nucleoli. The localization of PSF to speckles is dependent upon the presence of the second RRM motif. PSF also can associate with the DNA binding domains (DBDs) of thyroid hormone receptors and retinoic acid receptors, where it acts as a repressor by recruiting HDACs to the DBDs. PSF is expressed in neurons during development and may be involved in neuronal differentiation and maturation. PSF is proteolytically cleaved to produce a shorter fragment in myeloid cells. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| recommended for detection of PSF of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, canine, bovine and avian | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 581-660 mapping near the C-terminus of PSF of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 581-660 mapping near the C-terminus of PSF of human origin |
| Entrez Gene | 6421 |
|---|
Application
Immunofluorescence staining of formalin-fixed Hep G2 cells showing nuclear localization.
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human cerebral cortex tissue showing nuclear staining of neuronal and non-neuronal cells at low (A) and high (B) magnification. Kindly provided by The Swedish Human Protein Atlas (HPA) program.
Application
Western blot analysis of PSF expression in HeLa (A), A-431 (B), NIH/3T3 (C), 3611-RF (D), Hep G2 (E) and A-673 (F) nuclear extracts.| Application Notes | recommended for detection of PSF of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, canine, bovine and avian |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
