Application
Western blot analysis of β ig-h3 expression in non-transfected: (A) and human β ig-h3 transfected: (B) 293T whole cell lysates.Application
Immunofluorescence staining of methanol-fixed HeLa cells showing cytoplasmic localization.| Product Name | β ig-h3 Antibody (H-58) |
|---|---|
| Antibody Type | Primary Antibodies |
| Modification Notes | Human ∫ ig-h3 (alpha-3/beta-1 integrin, keratoepithelin) is a secreted, 683-amino acid, transforming growth factor-inducible, extracellular matrix adhesion molecule (1,2). ∫ ig-h3 contains an amino-terminal secretory sequence and a carboxy-terminal integrin-binding Arg-Gly-Asp (RGD) domain (2). ∫ ig-h3 is implicated in mechanisms leading to proliferation, differentiation, wound healing, and morphogenesis of corneal tissues (3). Mutations in the ∫ ig-h3 gene, along with elevated levels of ∫ ig-h3 protein in human corneas, occurs with granular dystrophy (GCD) and other inherited disorders of the cornea (4,5). ∫ ig-h3 is also a structural component of the human bladder extracellular matrix and may influence nuclear regulatory or structural functions (6). |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of &beta ig-h3 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including bovine | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 626-683 mapping at the C-terminus of &beta ig-h3 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 626-683 mapping at the C-terminus of β ig-h3 of human origin |
| Entrez Gene | 7045 |
|---|
Application
Western blot analysis of β ig-h3 expression in non-transfected: (A) and human β ig-h3 transfected: (B) 293T whole cell lysates.
Application
Immunofluorescence staining of methanol-fixed HeLa cells showing cytoplasmic localization.| Application Notes | recommended for detection of &beta ig-h3 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including bovine |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
