| Product Name | Dia 1 Antibody (H-58) |
|---|---|
| Antibody Type | Primary Antibodies |
| Modification Notes | Dia 1 (also designated p140mDia) and Dia 2 (also designated p134mDia 2), mammalian homologs of Drosophila Diaphanous, belong to a family of formin-homology (FH) proteins, which are characterized by having tandemly aligned FH1 and FH2 domains in their carboxy terminal regions (1-4). The amino terminus of inactivated Dia 1 binds to the FH1 and FH2 domains and is activated when GTP-bound Rho disrupts the intramolecular interaction of Dia 1 and converts it to an open conformation (1). Once activated, Dia 1 colocalizes with profilin and RhoA in dynamic plasma-membrane structures and induces the formation of stress fibers in conjunction with the ROCK mediated signaling pathway (12,4-6). However, Dia 1 and ROCK activities antagonize each other in order to determine the thickness and density of the stress fibersformed (1,6). Dia 1 has also been implicated as the gene responsible for non-syndromic deafness (1,7). |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of Dia 1 of mouse, rat and human origin by WB, IP, IF and ELISA | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 1191-1248 mapping at the C-terminus of diaphanous 1 (Dia 1) of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 1191-1248 mapping at the C-terminus of diaphanous 1 (Dia 1) of human origin |
| Entrez Gene | 1729 |
|---|
| Application Notes | recommended for detection of Dia 1 of mouse, rat and human origin by WB, IP, IF and ELISA |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
