Application
Western blot analysis of RasGRP1 expression in non-transfected: (A) and human RasGRP1 transfected: (B) 293T whole cell lysates.Application
Western blot analysis of RasGRP1 expression in Jurkat whole cell lysate.Application
Western blot analysis of human recombinant RasGRP1 fusion protein.| Product Name | RasGRP1 Antibody (H-120) |
|---|---|
| Antibody Type | Primary Antibodies |
| Modification Notes | The superfamily of GTP-binding proteins, of which Ras proteins are prototypes, has been implicated in a broad range of biological activities. Studies have identified a family of guanine nucleotide-releasing factors (GRFs) that activate Ras in mammalian cells and an “adapter” protein (Sem 5/GRB2) that appears to mediate the interaction of GRFs with activated receptor molecules (1-3). Subsequent to activation, Ras appears to interact with Raf, thereby activating the MAP kinase phosphorylation pathway (4). RasGRP is a guanyl nucleotide-releasing protein for Ras that contains two EF hand domains, which bind to calcium, and a diacylglycerol (DAG)-binding domain. RasGRP is expressed in the nervous system and lymphoid tissues and may link changes in DAG and calcium concentrations to Ras activation (5,6). |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of RasGRP1 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine and porcine | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 678-797 mapping at the C-terminus of RasGRP1 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 678-797 mapping at the C-terminus of RasGRP1 of human origin |
| Entrez Gene | 10125 |
|---|
Application
Western blot analysis of RasGRP1 expression in non-transfected: (A) and human RasGRP1 transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of RasGRP1 expression in Jurkat whole cell lysate.
Application
Western blot analysis of human recombinant RasGRP1 fusion protein.| Application Notes | recommended for detection of RasGRP1 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine and porcine |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
