Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human salivary gland tissue showing nuclear and cytoplasmic staining of glandular cells.Application
Western blot analysis of MDMX expression in non-transfected: (A) and human MDMX transfected: (B) 293T whole cell lysates.Application
Western blot analysis of MDMX expression in U-2 OS whole cell lysate.| Product Name | MDMX Antibody (H-130) |
|---|---|
| Antibody Type | Primary Antibodies |
| Modification Notes | The MDM2 protein is the primary regulator of p53 protein stability. MDMX is an MDM2-related protein that inhibits MDM2-mediated degradation of p53 via distinct associations with MDM2. The gene that encodes MDMX (also designated MDM4) is a target for amplification in malignant gliomas. ARF interacts with MDMX to sequester MDMX within the nucleolus. This sequestration of MDMX by ARF results in an increase in p53 transactivation. In addition, expression of MDMX can reverse MDM2-targeted degradation of p53 while maintaining suppression of p53 transactivation. Like MDM2, MDMX also binds p73 and stabilizes the level of p73. Therefore, in striking contrast to p53, the half-life of p73 is increased by binding to MDM2. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| recommended for detection of MDMX of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, canine, bovine and porcine | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 361-490 mapping at the C-terminus of MDMX of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 361-490 mapping at the C-terminus of MDMX of human origin |
| Entrez Gene | 4194 |
|---|
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human salivary gland tissue showing nuclear and cytoplasmic staining of glandular cells.
Application
Western blot analysis of MDMX expression in non-transfected: (A) and human MDMX transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of MDMX expression in U-2 OS whole cell lysate.| Application Notes | recommended for detection of MDMX of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, canine, bovine and porcine |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
