Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human bronchus tissue showing nuclear and cytoplasmic staining of respiratory epithelial cells.Application
Western blot analysis of Smad2/3 expression in K-562 (A), U-937 (B) and C2C12 (C) whole cell lysates.Application
Immunofluorescence staining of methanol-fixed HeLa cells showing cytoplasmic localization.Application
Western blot analysis of Smad3 expression in non-transfected: (A) and mouse Smad3 transfected: (B) 293T whole cell lysates.Application
Western blot analysis of Smad3 expression in non-transfected: (A) and human Smad3 transfected: (B) 293T whole cell lysates.Application
Western blot analysis of Smad2 and Smad3 expression in K-562 whole cell lysate.| Product Name | Smad2/3 Antibody (FL-425) |
|---|---|
| Antibody Type | Primary Antibodies |
| Modification Notes | Smad proteins, the mammalian homologs of the Drosophila mothers against decapentaplegic (Mad), have been implicated as downstream effectors of TGF∫/BMP signaling. Smad1 (also designated Madr1 or JV4-1) and Smad5 are effectors of BMP-2 and BMP-4 function, while Smad2 (also designated Madr2 or JV18-1) and Smad3 are involved in TGF∫ and Activin-mediated growth modulation. Smad4 (also designated DPC4) has been shown to mediate all of the above activities through interaction with various Smad family members. Smad6 and Smad7 regulate the response to Activin/TGF∫ signaling by interfering with TGF∫-mediated phosphorylation of other Smad proteins. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ChIPELISAGSIFIHCIPWB |
| WB:1:100~1:500 IP:1:20~1:100 IF:1:5~1:20 IHC:1:10~1:50 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | raised against amino acids 1-425 representing full length Smad3 of human origin |
|---|---|
| Tissue Specificity | raised against amino acids 1-425 representing full length Smad3 of human origin |
| Entrez Gene | 4088 |
|---|
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human bronchus tissue showing nuclear and cytoplasmic staining of respiratory epithelial cells.
Application
Western blot analysis of Smad2/3 expression in K-562 (A), U-937 (B) and C2C12 (C) whole cell lysates.
Application
Immunofluorescence staining of methanol-fixed HeLa cells showing cytoplasmic localization.
Application
Western blot analysis of Smad3 expression in non-transfected: (A) and mouse Smad3 transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of Smad3 expression in non-transfected: (A) and human Smad3 transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of Smad2 and Smad3 expression in K-562 whole cell lysate.| Application Notes | WB:1:100~1:500 IP:1:20~1:100 IF:1:5~1:20 IHC:1:10~1:50 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
