Application
Western blot analysis of UBC expression in non-transfected: (A) and mouse UBC transfected: (B) 293T whole cell lysates.Application
Western blot analysis of Ub expression in 293T whole cell lysate.Application
Western blot analysis of Ub expression in non-transfected: (A) and human Ub transfected: (B) 293T whole cell lysates.Application
Western blot analysis of Ub expression in non-transfected: (A) and human Ub transfected: (B) 293 whole cell lysates.Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human placenta tissue showing nuclear staining in decidual cells and cytoplasmic and nuclear stainining in trophoblastic cells. Kindly provided by The Swedish Human Protein Atlas (HPA) program.Application
Western blot analysis of Ub expression in NIH/3T3 (A) and HeLa (B) whole cell lysates.Application
Immunoperoxidase staining of formalin-fixed, paraffin-embedded human breast tumor showing cytoplasmic and nuclear staining.Application
Western blot analysis of lysates from control Jurkat cells (A), Jurkat cells treated with the proteasome inhibitor Lactacystin showing accumulation of ubiquitinated proteins (B) and human recombinant Ub (C).| Product Name | Ub Antibody (FL-76) AC |
|---|---|
| Antibody Type | Primary Antibodies |
| Modification Notes | Ubiquitin (Ub) is among the most phylogenetically conserved proteins known. The primary function of ubiquitin is to clear abnormal, foreign and improperly folded proteins by targeting them for degradation by the 26S proteosome. This small, 76 amino acid protein can be covalently attached to cellular proteins via an isopeptide linkage between the carboxy terminal group of ubiquitin and lysine amino groups on the acceptor protein. For proteolysis to occur, ubiquitin oligomers must be assembled. Ubiquitin chains on proteolytic substrates are commonly found to have an isopeptide bridge between Lysine 48 of one ubiquitin molecule and the carboxy-terminus of a neighboring ubiquitin molecule. Ubiquitin also plays a role in regulating signal transduction cascades through the elimination inhibitory proteins, such as IkBa and p27. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| recommended for detection of ubiquitin, polyubiquitin and ubiquitinated proteins in multiple species by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, canine, bovine, porcine and avian | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 1-76 representing full length Ub of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 1-76 representing full length Ub of human origin |
| Entrez Gene | 6233 |
|---|
Application
Western blot analysis of UBC expression in non-transfected: (A) and mouse UBC transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of Ub expression in 293T whole cell lysate.
Application
Western blot analysis of Ub expression in non-transfected: (A) and human Ub transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of Ub expression in non-transfected: (A) and human Ub transfected: (B) 293 whole cell lysates.
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human placenta tissue showing nuclear staining in decidual cells and cytoplasmic and nuclear stainining in trophoblastic cells. Kindly provided by The Swedish Human Protein Atlas (HPA) program.
Application
Western blot analysis of Ub expression in NIH/3T3 (A) and HeLa (B) whole cell lysates.
Application
Immunoperoxidase staining of formalin-fixed, paraffin-embedded human breast tumor showing cytoplasmic and nuclear staining.
Application
Western blot analysis of lysates from control Jurkat cells (A), Jurkat cells treated with the proteasome inhibitor Lactacystin showing accumulation of ubiquitinated proteins (B) and human recombinant Ub (C).| Application Notes | recommended for detection of ubiquitin, polyubiquitin and ubiquitinated proteins in multiple species by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, canine, bovine, porcine and avian |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
