Application
Western blot analysis of Cdx2 expression in HeLa whole cell lysate.Application
Western blot analysis of Cdx2 expression in non-transfected (A) and human Cdx2 transfected (B) 293 whole cell lysates.Application
Immunoperoxidase staining of formalin-fixed, paraffin-embedded human breast carcinoma tissue showing nuclear and cytoplasmic localization.Application
Western blot analysis of Cdx2 expression in human placenta tissue extract.| Product Name | Cdx2 Antibody (K-21) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | recommended for detection of Cdx2 of human origin by WB, IP, IF, IHC(P) and ELISA |
| Modification Notes | The members of the murine Cdx family (Cdx1, Cdx2, and Cdx4) are members of the caudal-type homeobox family of genes, which are homologues of the Drosophila ‘caudal’ gene required for anterior-posterior regional identity (1). The intestine-specific transcription factors Cdx1 and Cdx2 are candidate genes for directing intestinal development, differentiation, proliferation and maintenance of the intestinal phenotype (2,3). The relative expression of Cdx1 to Cdx2 protein may be important in the anterior to posterior patterning of the intestinal epithelium and in defining patterns of proliferation and differentiation along the crypt-villus axis (2). Expression of the Cdx1 homeobox gene in epithelial intestinal cells promotes cellular growth and differentiation (4). Cdx1 positively regulates its own expression (5). Cdx1 and Cdx2 are expressed in the small intestine and colon of fetus and adult (6). A decrease in human Cdx1 and/or Cdx2 expression is associated with colorectal tumorigenesis (7). Both Cdx1 and Cdx2 genes must be expressed to reduce tumorigenic potential, to increase sensitivity to apoptosis, and to reduce cell migration, suggesting that the two genes control the normal phenotype by independent pathways (8). The human Cdx1 gene maps to chromosome 5q32 and encodes a 265-amino acid protein (9). |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| WB:1:500~1:2000 IP:1:100~1:500 IF:1:200~1:500 IHC:1:50~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | recommended for detection of Cdx2 of human origin by WB IP IF IHC(P) and ELISA |
|---|---|
| Tissue Specificity | recommended for detection of Cdx2 of human origin by WB, IP, IF, IHC(P) and ELISA |
| Entrez Gene | 1045 |
|---|
Application
Western blot analysis of Cdx2 expression in HeLa whole cell lysate.
Application
Western blot analysis of Cdx2 expression in non-transfected (A) and human Cdx2 transfected (B) 293 whole cell lysates.
Application
Immunoperoxidase staining of formalin-fixed, paraffin-embedded human breast carcinoma tissue showing nuclear and cytoplasmic localization.
Application
Western blot analysis of Cdx2 expression in human placenta tissue extract.| Application Notes | WB:1:500~1:2000 IP:1:100~1:500 IF:1:200~1:500 IHC:1:50~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
