Application
Western blot analysis of RBQ-3 expression in non-transfected: (A) and human RBQ-3 transfected: (B) 293T whole cell lysates.Application
Western blot analysis of RBQ-3 expression in non-transfected: (A) and human RBQ-3 transfected: (B) 293T whole cell lysates.Application
Western blot analysis of RBQ-3 expression in HeLa whole cell lysate.| Product Name | RBQ-3 Antibody (H-269) |
|---|---|
| Antibody Type | Primary Antibodies |
| Modification Notes | WD-repeats are motifs that are found in a variety of proteins and are characterized by a conserved core of 40-60 amino acids that commonly form a tertiary propeller structure. While proteins that contain WD-repeats participate in a wide range of cellular functions, they are generally involved in regulatory mechanisms concerning chromatin assembly, cell cycle control, signal transduction, RNA processing, apoptosis and vesicular trafficking. RBQ-3, also known as RBBP5 (retinoblastoma binding protein 5) or SWD1, is a 538 amino acid protein that localizes to the nucleus and contains six WD repeats. Expressed ubiquitously, RBQ-3 functions as a component of the Set1 complex and preferentially binds to underphosphorylated forms of the retinoblastoma (Rb) protein, possibly playing a role in the regulation of cell proliferation. RBQ-3 exists as two alternatively spliced isoforms and, upon DNA damage, is subject to phosphorylation by ATM or ATR. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of RBQ-3 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine, porcine and avian | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 270-538 mapping at the C-terminus of RBQ-3 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 270-538 mapping at the C-terminus of RBQ-3 of human origin |
| Entrez Gene | 5929 |
|---|
Application
Western blot analysis of RBQ-3 expression in non-transfected: (A) and human RBQ-3 transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of RBQ-3 expression in non-transfected: (A) and human RBQ-3 transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of RBQ-3 expression in HeLa whole cell lysate.| Application Notes | recommended for detection of RBQ-3 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine, porcine and avian |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
