| Product Name | KCC2a Antibody (E-13) |
|---|---|
| Antibody Type | Primary Antibodies |
| Modification Notes | Nitric oxide (NO) donors activate KCCs, while inhibitors of the cGMP pathway prevent NO donor activation. KCC2 expression is limited to neuronal tissues by a restrictive element similar to the neuronal-restrictive silencing factor. In neurons, KCC2 expression is correlated with an inhibitory response to GABA, while the absence of KCC2 is necessary for an unusual excitatory response to GABA. Alterations of KCC2 expression in the inferior colliculus of rat brain may be related to seizure susceptibility. KCC2a, also known as SLC12A5 (solute carrier family 12 member 5), is a 1,139 amino acid multi-pass membrane protein that belongs to the SLC12A transporter family. Detected in neuronal cells, KCC2a is brain specific and may mediate electroneutral potassium-chloride cotransport. KCC2a is inhibited by furosemide and bumetanide, and exists as a homomultimer and heteromultimer with other K-Cl cotransporters. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIFELISA |
| recommended for detection of Isoform KCC2a of mouse, rat and human origin by WB, IF and ELISA non cross-reactive with isoform KCC2b non cross-reactive with KCC1, KCC3 or KCC4 | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope mapping within an N-terminal cytoplasmic domain of KCC2 of human origin |
|---|---|
| Tissue Specificity | epitope mapping within an N-terminal cytoplasmic domain of KCC2 of human origin |
| Entrez Gene | 57468 |
|---|
| Application Notes | recommended for detection of Isoform KCC2a of mouse, rat and human origin by WB, IF and ELISA non cross-reactive with isoform KCC2b non cross-reactive with KCC1, KCC3 or KCC4 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
