Application
Immunofluorescence staining of normal mouse intestine frozen section showing cytoplasmic staining.Application
PKC antibodies used include PKC α (C-20), PKC βI (C-16), PKC βII (C-18), PKC γ (C-19), PKC ε (C-15), PKC ζ (C-20) and PKC η (C-15).Application
Immunofluorescence staining of methanol-fixed HeLa cells.Application
Antibodies tested include PKC α (C-20): (A), PKC (MC5): (B) and PKC α (H-7): (C).Application
Western blot analysis of PKC α expression in Jurkat (A) and NIH/3T3 (B) whole cell lysates and rat brain extract (C).| Product Name | PKC α Antibody (C-20) |
|---|---|
| Antibody Type | Primary Antibodies |
| Modification Notes | Members of the protein kinase C (PKC) family play a key regulatory role in a variety of cellular functions including cell growth and differentiation, gene expression, hormone secretion and membrane function. PKCs were originally identified as serine/threonine protein kinases whose activity was dependent on calcium and phospholipids. Diacylglycerols (DAG) and tumor-promoting phorbol esters bind to and activate PKC. PKCs can be subdivided into many different isoforms (å, ∫I, ∫II, ©, ∂, é, Ω, h, †, λ/î, µ and n). Patterns of expression for each PKC isoform differ among tissues and PKC family members exhibit clear differences in their cofactor dependencies. For instance, the kinase activities of PKC ∂ and é are independent of Ca2+. On the other hand, most of the other PKC members possess phorbol ester-binding activities and kinase activities. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISAChIP |
| recommended for detection of PKC &alpha and, to a lesser extent, PKC &betaI and PKC &gamma of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine and bovine | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope mapping at the C-terminus of PKC &alpha of human origin |
|---|---|
| Tissue Specificity | epitope mapping at the C-terminus of PKC α of human origin |
| Entrez Gene | 5578 |
|---|
Application
Immunofluorescence staining of normal mouse intestine frozen section showing cytoplasmic staining.
Application
PKC antibodies used include PKC α (C-20), PKC βI (C-16), PKC βII (C-18), PKC γ (C-19), PKC ε (C-15), PKC ζ (C-20) and PKC η (C-15).
Application
Immunofluorescence staining of methanol-fixed HeLa cells.
Application
Antibodies tested include PKC α (C-20): (A), PKC (MC5): (B) and PKC α (H-7): (C).
Application
Western blot analysis of PKC α expression in Jurkat (A) and NIH/3T3 (B) whole cell lysates and rat brain extract (C).| Application Notes | recommended for detection of PKC &alpha and, to a lesser extent, PKC &betaI and PKC &gamma of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine and bovine |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
