Application
Western blot analysis of Neutrophil Elastase expression in U-937 whole cell lysate.Application
Western blot analysis of human recombinant Neutrophil Elastase.Application
Immunofluorescence staining of methanol-fixed U-937 cells showing cytoplasmic localization.| Product Name | Neutrophil Elastase Antibody (H-57) |
|---|---|
| Antibody Type | Primary Antibodies |
| Modification Notes | Neutrophil Elastase (NE) is a serine protease that is expressed in bone marrow precursor cells, stored in peripheral blood granulocytes, and implicated in the progression of a variety of inflammatory diseases, including idiopathic pulmonary fibrosis, rheumatoid arthritis, adult respiratory distress syndrome and cystic fibrosis. In neutrophils, Neutrophil Elastase contributes largely to the proteolysis of phagocytosed proteins, the migration of neutrophils and the remodeling of tissues following injury. Neutrophil Elastase, which is also designated medullasin, is secreted into the extracellular matrix, where it is then capable of destroying connective tissue proteins, including elastin, proteoglycans and Type IV Collagens. Neutrophil Elastase also mediates proteolysis by cleaving proteins that are associated with the complement system, such as antithrombin and Fibrinogen. Additionally, Neutrophil Elastase functions as a potent platelet agonist, where it potentiates the aggregation, secretion and mobilization of calcium in response to cathepsin G binding to platelet surface receptors. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFIPWB |
| WB:1:100~1:500 IP:1:20~1:100 IF:1:5~1:20 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 211-267 mapping at the C-terminus of Neutrophil Elastase of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 211-267 mapping at the C-terminus of Neutrophil Elastase of human origin |
| Entrez Gene | 1991 |
|---|
Application
Western blot analysis of Neutrophil Elastase expression in U-937 whole cell lysate.
Application
Western blot analysis of human recombinant Neutrophil Elastase.
Application
Immunofluorescence staining of methanol-fixed U-937 cells showing cytoplasmic localization.| Application Notes | WB:1:100~1:500 IP:1:20~1:100 IF:1:5~1:20 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
