Application
Western blot analysis of MBL-C expression in c4 (A), Jurkat (B) and CCRF-CEM (C) whole cell lysates and rat liver tissue extract (D).Application
Western blot analysis of MBL-C expression in Hep G2 (A), KNRK (B) and DU 145 (C) whole cell lysates and rat kidney tissue extract (D).| Product Name | MBL-C Antibody (H-50) |
|---|---|
| Antibody Type | Primary Antibodies |
| Modification Notes | Mannose-binding lectin protein C (MBL-C), also known as mannose-binding protein C; mannose-binding lectin 2, soluble (opsonic defect); mannan-binding lectin; mannan-binding protein; and soluble mannose-binding lectin, initiates the lectin branch of the innate immune response by binding to the surface of potentially pathogenic microorganisms and initiating complement fixation through an N-terminal collagen-like domain. MBL-C is a key component in immune response due to its ability to directly trigger neutralization of invading microorganisms by an Ab-independent mechanism. It binds to sugars on the surface of bacterial, fungal and parasitic cells through C-terminal, Ca2+-dependent carbohydrate-recognition domains. Mutations of human MBL are associated with immunodeficiency resulting from a reduction in the ability of the mutant MBL to initiate complement fixation. In human, two types of MBL-associated serine proteases (MASP-1 and MASP-2) and a truncated form of MASP-2, designated small MBL-associated protein (sMAP) or MAp19, complex with MBL to activate the lectin pathway of the complement system. Activated MASPs subsequently cleave and activate downstream components of the complement pathway. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of MBL of human origin by WB, IP, IF and ELISA | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 101-150 mapping to an internal region of MBL-C-C of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 101-150 mapping to an internal region of MBL-C-C of human origin |
| Entrez Gene | 4153 |
|---|
Application
Western blot analysis of MBL-C expression in c4 (A), Jurkat (B) and CCRF-CEM (C) whole cell lysates and rat liver tissue extract (D).
Application
Western blot analysis of MBL-C expression in Hep G2 (A), KNRK (B) and DU 145 (C) whole cell lysates and rat kidney tissue extract (D).| Application Notes | recommended for detection of MBL of human origin by WB, IP, IF and ELISA |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
