Application
Immunofluorescence staining of methanol-fixed HeLa cells showing nuclear localization.Application
Western blot analysis of SSB-4 expression in non-transfected: (A) and mouse SSB-4 transfected: (B) 293T whole cell lysates.| Product Name | SSB-4 Antibody (E-13) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope mapping near the N-terminus of SSB-4 of human origin |
| Modification Notes | Members of the suppressor of cytokine signaling (SOCS) family of proteins contain C-terminal regions of homology called the SOCS box, which serves to couple SOCS proteins and their binding partners with the Elongin BC complex, thereby mediating protein degradation. Several other families of proteins also contain SOCS boxes, but differ from the SOCS proteins in the type of domain they contain upstream of the SOCS box. SSB-4, also known as SPSB4 (splA/ryanodine receptor domain and SOCS box containing 4), is a 273 amino acid protein that localizes to the cytoplasm and contains one SOCS box domain and one B30.2/SPRY domain. Functioning as a substrate recognition component of the Elongin BC complex, SSB-4 plays a role in the ubiquitination and subsequent proteasomal degradation of target proteins. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of SSB-4 of mouse, rat and human origin by WB, IP, IF and ELISA non cross-reactive with SSB-1, SSB-2 or SSB-3: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope mapping near the N-terminus of SSB-4 of human origin |
|---|---|
| Tissue Specificity | epitope mapping near the N-terminus of SSB-4 of human origin |
| Entrez Gene | 92369 |
|---|
Application
Immunofluorescence staining of methanol-fixed HeLa cells showing nuclear localization.
Application
Western blot analysis of SSB-4 expression in non-transfected: (A) and mouse SSB-4 transfected: (B) 293T whole cell lysates.| Application Notes | recommended for detection of SSB-4 of mouse, rat and human origin by WB, IP, IF and ELISA non cross-reactive with SSB-1, SSB-2 or SSB-3: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
