Application
Western blot analysis of β-SNAP expression in non-transfected: (A) and mouse β-SNAP transfected: (B) 293T whole cell lysates.| Product Name | β-SNAP Antibody (E-13) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope mapping near the N-terminus of β-SNAP of human origin |
| Modification Notes | Syntaxins were originally thought to be docking proteins, but have more recently been categorized as anchoring proteins that anchor themselves to the cytoplasmic surfaces of cellular membranes. Syntaxins have been shown to bind to various proteins involved in exocytosis, including VAMPs (vesicle-associated membrane proteins), NSF (N-ethylmaleimide-sensitive factor), SNAP 25 (synaptosomal-associated protein of 25kDa), SNAPs (soluble NSF attachment proteins) and synaptotagmin. VAMPs (also designated synaptobrevins), including VAMP-1 and VAMP-2, and synaptotagmin, a protein that may function as an inhibitor of exocytosis, are vesicular proteins. SNAPs, including å-SNAP, ∫-SNAP and ©-SNAP, are cytoplasmic proteins that bind to a membrane receptor complex composed of VAMP, SNAP 25 and syntaxin. While å-SNAP and ©-SNAP, are found in a wide range of tissues, ∫-SNAP is expressed only in the brain. The gene encoding ∫-SNAP is localized to chromosome 20p11.21. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of β-SNAP of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including canine, bovine, porcine and avian: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope mapping near the N-terminus of &beta -SNAP of human origin |
|---|---|
| Tissue Specificity | epitope mapping near the N-terminus of β-SNAP of human origin |
| Entrez Gene | 63908 |
|---|
Application
Western blot analysis of β-SNAP expression in non-transfected: (A) and mouse β-SNAP transfected: (B) 293T whole cell lysates.| Application Notes | recommended for detection of β-SNAP of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including canine, bovine, porcine and avian: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
