Application
Immunofluorescence staining of methanol-fixed HeLa cells showing cytoplasmic localization.Application
Western blot analysis of CaM expression in rat brain tissue extract.Application
Western blot analysis of CaM expression in mouse brain tissue extract.Application
Western blot analysis of CALML3 expression in non-transfected: (A) and human CALML3 transfected: (B) 293T whole cell lysates.Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human prostate tissue showing cytoplasmic and membrane staining of glandular cells (low and high magnification). Kindly provided by The Swedish Human Protein Atlas (HPA) program.Application
Western blot analysis of CALML3 expression in non-transfected: (A) and human CALML3 transfected: (B) 293T whole cell lysates.Application
Western blot analysis of CaM expression in rat skeletal muscle extract.Application
Immunofluorescence staining of methanol-fixed SJRH30 cells showing membrane and cytoplasmic localization.| Product Name | CaM Antibody (FL-149) AC |
|---|---|
| Antibody Type | Primary Antibodies |
| Modification Notes | The level of intracellular calcium is tightly regulated in all eukaryotic cells. A modest increase in this level can result in a myriad of physiological responses, most of which are mediated by calmodulin (CaM), the universal calcium sensor. CaM directly modulates the activity of protein kinases and phosphatases, ion channels and nitric oxide synthetases. It is generally involved in such diverse processes as cell proliferation, endocytosis, cellular adhesion, protein turn over and smooth muscle contraction. CaM (calmodulin) is an acidic protein, 148 amino acids in length, with four helix-loop-helix calcium binding domains. In humans, 3 distinct genes have been identified (CALM1, CALM2 and CALM3); each encoding the identical protein. CALML3 (calmodulin-like 3, or calmodulin-related protein NB-1) shares significant sequence identity with CaM and it is suggested that it may competitively bind CaM substrates. Interestingly, CaM has been shown to associate with the carboxy terminus of the dystrophin gene product, implying that it may regulate its activity. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| recommended for detection of calmodulin and CALML3 (calmodulin-like 3) of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, canine, bovine, porcine and avian | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 1-149 representing full length Calmodulin (CaM I) of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 1-149 representing full length Calmodulin (CaM I) of human origin |
| Entrez Gene | 801 |
|---|
Application
Immunofluorescence staining of methanol-fixed HeLa cells showing cytoplasmic localization.
Application
Western blot analysis of CaM expression in rat brain tissue extract.
Application
Western blot analysis of CaM expression in mouse brain tissue extract.
Application
Western blot analysis of CALML3 expression in non-transfected: (A) and human CALML3 transfected: (B) 293T whole cell lysates.
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human prostate tissue showing cytoplasmic and membrane staining of glandular cells (low and high magnification). Kindly provided by The Swedish Human Protein Atlas (HPA) program.
Application
Western blot analysis of CALML3 expression in non-transfected: (A) and human CALML3 transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of CaM expression in rat skeletal muscle extract.
Application
Immunofluorescence staining of methanol-fixed SJRH30 cells showing membrane and cytoplasmic localization.| Application Notes | recommended for detection of calmodulin and CALML3 (calmodulin-like 3) of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, canine, bovine, porcine and avian |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
