Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human adrenal gland tissue showing cytoplasmic staining of glandular cells.Application
Western blot analysis of Flg expression in K-562 whole cell lysate.Application
Western blot analysis of Flg expression in non-transfected: (A) and mouse Flg transfected: (B) 293T whole cell lysates.Application
Western blot analysis of Flg expression in non-transfected: (A) and human Flg transfected: (B) 293T whole cell lysates.Application
Western blot analysis of Flg expression in MOLT-4 (A) and WI 38 (B) whole cell lysates.Application
Immunoperoxidase staining of formalin-fixed, paraffin-embedded human breast carcinoma tissue showing cytoplasmic and membrane staining (A). Immunofluorescence staining of methanol-fixed HeLa cells. Note specific cytoplasmic fluorescein immunostaining and nuclear DAPI counterstain (B).| Product Name | Flg Antibody (C-15) |
|---|---|
| Antibody Type | Primary Antibodies |
| Modification Notes | Acidic and basic fibroblast growth factors (FGFs) are members of a family of multifunctional polypeptide growth factors that stimulate proliferation of cells of mesenchymal, epithelial and neuroectodermal origin. Like other growth factors, FGFs act by binding and activating specific cell surface receptors. These include the Flg receptor (FGFR-1), the Bek receptor (FGFR-2), FGFR-3, FGFR-4, FGFR-5 and FGFR-6. These receptors usually contain an extracellular ligand-binding region containing three immunoglobulin-like domains, a transmembrane domain and a cytoplasmic tyrosine kinase domain. The gene encoding human Flg maps to chromosome 8p12 and is alternatively spliced to produce several isoforms. Mutations in Flg are associated with Pfeiffer syndrome (a skeletal disorder characterized by craniosynostosis with deviation and enlargement of the thumbs and great toes), brachymesophalangy with phalangeal ankylosis and a varying degree of soft tissue syndactyly. The Flg gene is also involved in chromosomal translocations with ZNF198, CEP110 and FOP, which may lead to stem cell leukemia lymphoma (SCLL). |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| recommended for detection of Flg of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, canine, bovine and porcine | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope mapping at the C-terminus of Flg of human origin |
|---|---|
| Tissue Specificity | epitope mapping at the C-terminus of Flg of human origin |
| Entrez Gene | 2260 |
|---|
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human adrenal gland tissue showing cytoplasmic staining of glandular cells.
Application
Western blot analysis of Flg expression in K-562 whole cell lysate.
Application
Western blot analysis of Flg expression in non-transfected: (A) and mouse Flg transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of Flg expression in non-transfected: (A) and human Flg transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of Flg expression in MOLT-4 (A) and WI 38 (B) whole cell lysates.
Application
Immunoperoxidase staining of formalin-fixed, paraffin-embedded human breast carcinoma tissue showing cytoplasmic and membrane staining (A). Immunofluorescence staining of methanol-fixed HeLa cells. Note specific cytoplasmic fluorescein immunostaining and nuclear DAPI counterstain (B).| Application Notes | recommended for detection of Flg of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, canine, bovine and porcine |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
