Application
Western blot analysis of PAOX expression in human fetal muscle tissue extract.Application
Western blot analysis of PAOX expression in non-transfected: (A) and human PAOX transfected: (B) 293T whole cell lysates.Application
Western blot analysis of PAOX expression in non-transfected: (A) and mouse PAOX transfected: (B) 293T whole cell lysates.| Product Name | PAOX Antibody (A-21) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | recommended for detection of PAOX of mouse and human origin by WB, IP and ELISA |
| Modification Notes | PAOX (polyamine oxidase (exo-N4-amino)), also known as PAO, is a 649 amino acid protein that localizes to both the cytoplasm and to peroxisomes and belongs to the flavin monoamine oxidase family. Expressed in a variety of tissues, PAOX exists as a monomer that uses FAD as a cofactor to catalyze the oxidation of N(1)-acetylspermine and N(1)-acetylspermidine to spermidine and putrescine, respectively, thereby playing a role in polyamine back-conversion, as well as in the regulation of polyamine intracellular concentration. Multiple isoforms of PAOX exist due to alternative splicing events. The gene encoding PAOX maps to human chromosome 10, which houses over 1,200 genes and comprises nearly 4.5% of the human genome. Defects in some of the genes that map to chromosome 10 are associated with Charcot-Marie Tooth disease, Jackson-Weiss syndrome, Usher syndrome, nonsyndromatic deafness, Wolman’s syndrome, Cowden syndrome, multiple endocrine neoplasia type 2 and porphyria. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPELISA |
| WB:1:500~1:2000 IP:1:100~1:500 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | recommended for detection of PAOX of mouse and human origin by WB IP and ELISA |
|---|---|
| Tissue Specificity | recommended for detection of PAOX of mouse and human origin by WB, IP and ELISA |
| Entrez Gene | 196743 |
|---|
Application
Western blot analysis of PAOX expression in human fetal muscle tissue extract.
Application
Western blot analysis of PAOX expression in non-transfected: (A) and human PAOX transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of PAOX expression in non-transfected: (A) and mouse PAOX transfected: (B) 293T whole cell lysates.| Application Notes | WB:1:500~1:2000 IP:1:100~1:500 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
