Application
Western blot analysis of NKD2 expression in Jurkat whole cell lysate.| Product Name | NKD2 Antibody (L-24) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | recommended for detection of NKD2 of h and r origin by WB, IP and ELISA |
| Modification Notes | The canonical Wnt signaling pathway is a complex network of proteins involved in binding the Wnt ligand to the Frizzled family of receptors, leading to activation of the Dvl proteins and, ultimately, a change in ∫-catenin concentration in the nucleus. NKD2 (naked cuticle homolog 2), also known as Naked2, is a 451 amino acid cell membrane protein belonging to the NKD family. Through interactions with Dvl-1, Dvl-2, Dvl-3 and PP2A-B72/B130, NKD2 functions as an autonomous antagonist of the classical Wnt signaling pathway and activates a second Wnt signaling pathway that controls planar cell polarity. NKD2 is required for processing of TGFå and for escorting TGFå to the basolateral membrane of polarized epithelial cells. NKD2 is a homolog of Drosophila Naked Cuticle, which negatively regulates canonical Wnt signaling by binding dishevelled. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPELISA |
| WB:1:500~1:2000 IP:1:100~1:500 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | recommended for detection of NKD2 of h and r origin by WB IP and ELISA |
|---|---|
| Tissue Specificity | recommended for detection of NKD2 of h and r origin by WB, IP and ELISA |
| Entrez Gene | 85409 |
|---|
Application
Western blot analysis of NKD2 expression in Jurkat whole cell lysate.| Application Notes | WB:1:500~1:2000 IP:1:100~1:500 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
