Application
Western blot analysis of TRIM35 expression in Hep G2 whole cell lysate.| Product Name | TRIM35 Antibody (H-21) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | recommended for detection of TRIM35 of human origin by WB, IP and ELISA |
| Modification Notes | TRIM35 (tripartite motif-containing 35), also known as HLS5 (hemopoietic lineage switch protein 5) or MAIR (macrophage-derived apoptosis-inducing RBCC protein), is a widely expressed 493 amino acid protein that belongs to the TRIM/RBCC (Ring finger, B box, coiled-coil) family. TRIM35 contains a B box-type zinc finger, a coiled-coil domain, a SPRY domain and a RING-type zinc finger; a motif that has zinc-chelating activity and is involved in mediating protein-protein and protein-DNA interactions. Localizing to cytoplasmic granules and punctate nuclear bodies, TRIM35 is believed to play a role in the cell death mechanism. The forced expression of TRIM35 in HeLa cells results in the inhibition of tumorigenicity, cell growth and clonogenicity. In addition, the gene encoding TRIM35 localizes to a region of chromosome 8 that has been implicated in a number of leukemias and solid tumors. This suggests that TRIM35 may function as a tumor suppressor. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPELISA |
| WB:1:500~1:2000 IP:1:100~1:500 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | recommended for detection of TRIM35 of human origin by WB IP and ELISA |
|---|---|
| Tissue Specificity | recommended for detection of TRIM35 of human origin by WB, IP and ELISA |
| Entrez Gene | 23087 |
|---|
Application
Western blot analysis of TRIM35 expression in Hep G2 whole cell lysate.| Application Notes | WB:1:500~1:2000 IP:1:100~1:500 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
