Application
Western blot analysis of Bestrophin-4 expression in Hep G2 whole cell lysate.| Product Name | Bestrophin-4 Antibody (O-21) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | recommended for detection of Bestrophin-4 of human origin by WB, IP and ELISA; also reactive with additional species, including canine |
| Modification Notes | Bestrophin-4, also known as BEST4 or VMD2L2 (Vitelliform macular dystrophy 20-like protein 2), is a 473 amino acid member of the Bestrophin family of proteins. Members of the Bestrophin family are transmembrane proteins that contain a high percentage of aromatic residues, a conserved RFP (Arg-Phe-Pro) motif and they function as anion channels. Bestrophin-4 acts as a calcium-sensitive chloride channel located in the cell membrane. It is believed that Bestrophin-4 also acts as a channel for other physiologically significant anions, such as bicarbonate. Bestrophin-4 is predominantly expressed in the colon, but can be found at low levels in testis, placenta, trachea, spinal chord, lung and retina. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPELISA |
| WB:1:500~1:2000 IP:1:100~1:500 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | recommended for detection of Bestrophin-4 of human origin by WB IP and ELISA also reactive with additional species including canine |
|---|---|
| Tissue Specificity | recommended for detection of Bestrophin-4 of human origin by WB, IP and ELISA; also reactive with additional species, including canine |
| Entrez Gene | 266675 |
|---|
Application
Western blot analysis of Bestrophin-4 expression in Hep G2 whole cell lysate.| Application Notes | WB:1:500~1:2000 IP:1:100~1:500 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
