Application
Western blot analysis of HMG-20A expression in HeLa whole cell lysate.| Product Name | HMG-20A Antibody (X-24) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | recommended for detection of HMG-20A of mouse, rat and human origin by WB, IP and ELISA |
| Modification Notes | High mobility group (HMG) proteins 1 and 2 are ubiquitous non-histone components of chromatin. The binding of HMG proteins to the minor groove of AT-rich DNA sequences induces alterations in the DNA architecture, including DNA bending and unwinding of the helix. While HMG proteins do not stimulate initiation of transcription, they do enhance the binding of other transcription factors, such as Oct-2, members of the NF˚B family, ATF-2 and c-Jun, to activate transcription. HMG-20A (high-mobility group 20A), also known as HMGX1 or HMGXB1, is a 347 amino acid protein that localizes to the nucleus and contains one HMG box DNA-binding domain. Expressed ubiquitously, HMG-20A functions as an inhibitor of BRAF35 and, via its association with DNA, plays a role in neuronal differentiation and may also mediate the methylation of Histone H3. Multiple isoforms of HMG-20A exist due to alternative splicing events. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPELISA |
| WB:1:500~1:2000 IP:1:100~1:500 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | recommended for detection of HMG-20A of mouse rat and human origin by WB IP and ELISA |
|---|---|
| Tissue Specificity | recommended for detection of HMG-20A of mouse, rat and human origin by WB, IP and ELISA |
| Entrez Gene | 10363 |
|---|
Application
Western blot analysis of HMG-20A expression in HeLa whole cell lysate.| Application Notes | WB:1:500~1:2000 IP:1:100~1:500 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
