Application
Western blot analysis of V5-tagged fusion protein in non-transfected: (A) and V5-tagged human karyopherin 6 transfected: (B) 293T whole cell lysates.| Product Name | V5-Probe Antibody (G-14) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope mapping within an internal region of proteins P and V of Simian Virus 5 origin |
| Modification Notes | Plasmid vectors for the expression of coding regions of eukaryotic genes in bacterial, insect and mammalian hosts are in common usage; such expression vectors are frequently used to encode hybrid fusion proteins consisting of a eukaryotic target protein and a specialized region designed to aid in the purification and visualization of the target protein. An example is the V5-Probe which recognizes a small epitope, termed Pk, on the P/V proteins of the paramyxovirus simian virus 5 (SV5). This small peptide has proven useful in visualization and immunoaffinity purification of expressed fusion proteins. More than 20 recombinant proteins, some of which include transmembrane and secreted proteins, have been tagged with this epitope and detected via western blot, immunoprecipitation and immunofluorescence. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of V5 fusion proteins by WB, IP, IF and ELISA: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope mapping within an internal region of proteins P and V of Simian Virus 5 origin |
|---|---|
| Tissue Specificity | epitope mapping within an internal region of proteins P and V of Simian Virus 5 origin |
Application
Western blot analysis of V5-tagged fusion protein in non-transfected: (A) and V5-tagged human karyopherin 6 transfected: (B) 293T whole cell lysates.| Application Notes | recommended for detection of V5 fusion proteins by WB, IP, IF and ELISA: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
