Application
Western blot analysis of KLF6 expression in non-transfected: (A) and human KLF6 transfected: (B) 293T whole cell lysates.Application
Western blot analysis of KLF6 expression in non-transfected: (A) and mouse KLF6 transfected: (B) 293T whole cell lysates.Application
Immunoperoxidase staining of formalin-fixed, paraffin-embedded human liver carcinoma tissue showing nuclear localization.| Product Name | KLF6 Antibody (R-173) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 28-201 of Zf9 of rat origin |
| Modification Notes | The Krueppel Zinc finger transcription factor was initially identified in Drosophila as a segmentation gene. The mammalian family of Krueppel-type zinc finger transcription factors comprise a conserved family of DNA-binding proteins that are important in developmental regulation. The Krueppel-like factor 6 (KLF6) protein is a nuclear DNA-binding protein. KLF6 reduces cell proliferation by upregulating p21 in a p53-independent manner. KLF6 is also known as transcription factor ZF9, B cell derived 1 (BCD1), and core promoter element-binding protein (COPEB). KLF6 is predominantly expressed in the placenta but is also present in spleen, thymus, prostate, testis, small intestine and colon. In placenta, KLF6, KLF4 and pregnancy glycoprotein are co-expressed in the same cell types of placenta villi and membranes. The gene encoding human KLF6 maps to chromosome 10p15, and it is mutated in a subset of human prostate cancer. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISAGSChIP |
| recommended for detection of KLF6 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA may cross-react with KLF7 also reactive with additional species, including equine, canine, bovine and porcine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 28-201 of Zf9 of rat origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 28-201 of Zf9 of rat origin |
| Entrez Gene | 1316 |
|---|
Application
Western blot analysis of KLF6 expression in non-transfected: (A) and human KLF6 transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of KLF6 expression in non-transfected: (A) and mouse KLF6 transfected: (B) 293T whole cell lysates.
Application
Immunoperoxidase staining of formalin-fixed, paraffin-embedded human liver carcinoma tissue showing nuclear localization.| Application Notes | recommended for detection of KLF6 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA may cross-react with KLF7 also reactive with additional species, including equine, canine, bovine and porcine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
